Abstract
19-nor-progesterone (19-nor-P) has the characteristics of a potent mineralocorticoid in adrenalectomized or salt-loaded rats and is capable of causing hypertension. In human placenta, progesterone is converted to 19-hydroxy-progesterone, a precursor of 19-nor-P. In some states of pregnancy hypertension, 19-nor-P may inhibit renal 11 beta-hydroxysteroid dehydrogenase type 2 (11 beta-HSD2), thus allowing cortisol to bind to the mineralocorticoid receptor (MR). Therefore, we investigated the ability of 19-nor-P to inhibit human 11 beta-HSD2. Fetal kidney cells (HEK 293) were transfected with human 11 beta-HSD2 and incubated with increasing concentrations of 19-nor-P, labelled and unlabelled cortisol. Steroids were extracted, separated by TLC, and radioactivity was measured using a TLC scanner. 19-nor-P treatment did not significantly reduce 11 beta-HSD2 activity (430 to 300 pmol/mg protein/h) in the range of tested concentrations. In conclusion, 19-nor-P did not inhibit human 11 beta-HSD2 and seems not to be involved in human hypertension. Nevertheless, 19-nor-P may be converted by extra-adrenal tissues into 19-nor-deoxycorticosterone (DOC) or 19-nor-corticosterone, which are potent mineralocorticoids and may be involved in the pathogenesis of hypertension during pregnancy.
Original language | English |
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Pages (from-to) | 376-379 |
Number of pages | 4 |
Journal | Clinical and Experimental Hypertension |
Volume | 31 |
Issue number | 4 |
DOIs | |
Publication status | Published - 1 Jan 2009 |
Keywords
- mineralocorticoid receptor
- hypertension
- 19-nor-progesterone
- rat