The human germinal centre cells, follicular dendritic cells and germinal centre T cells produce B cell-stimulating cytokines

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The human germinal centre cells, follicular dendritic cells and germinal centre T cells produce B cell-stimulating cytokines. / Toellner, K M; Scheel-Toellner, D; Sprenger, R; Duchrow, M; Trümper, L H; Ernst, M; Flad, H D; Gerdes, J.

In: Cytokine, Vol. 7, No. 4, 05.1995, p. 344-54.

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Toellner, K M ; Scheel-Toellner, D ; Sprenger, R ; Duchrow, M ; Trümper, L H ; Ernst, M ; Flad, H D ; Gerdes, J. / The human germinal centre cells, follicular dendritic cells and germinal centre T cells produce B cell-stimulating cytokines. In: Cytokine. 1995 ; Vol. 7, No. 4. pp. 344-54.

Bibtex

@article{e9cf362c30b84edaa7a78e2baff9ae3d,
title = "The human germinal centre cells, follicular dendritic cells and germinal centre T cells produce B cell-stimulating cytokines",
abstract = "Cytokine gene expression was investigated in the germinal centre constituents of follicular dendritic cells and germinal centre T cells and compared to the mRNA expression of nongerminal centre tonsillar cells. Cells were isolated from human tonsils by preenrichment with MACS and subsequent FACS sorting. Cytokine gene expression was investigated by intronspanning RT-PCR for IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-10, IFN-alpha, IFN-beta, IFN-gamma, and TNF-alpha. Frequency of cytokine-producing cells and the cytokine production pattern of single cells were determined by single cell PCR. Furthermore, cytokine protein expression was investigated by immunohistology. Using these methods, we found a strong production of IL-1 beta mRNA and protein in a small percentage of FDC. Germinal centre T cells showed production of IL-2, IL-4, IL-10, IFN-gamma, and TNF-alpha mRNA. The mRNAs of these cytokines could also be detected at the single cell level; as they were produced in a high percentage of germinal centre T cells, whereas immunohistological staining was negative, we conclude that a high percentage of germinal centre T lymphocytes produce IL-2, IL-4, IL-10, IFN-gamma, and TNF-alpha in low concentrations in contrast to T cells outside the germinal centre, in which strong cytokine production in few cells was shown earlier.",
keywords = "Dendritic Cells, Tumor Necrosis Factor-alpha, Humans, Interferons, Interleukins, Child, Cell Separation, B-Lymphocytes, RNA, Messenger, Polymerase Chain Reaction, Base Sequence, Palatine Tonsil, Molecular Sequence Data, Cytokines, Flow Cytometry, Gene Expression Regulation, Immunoenzyme Techniques, T-Lymphocytes",
author = "Toellner, {K M} and D Scheel-Toellner and R Sprenger and M Duchrow and Tr{\"u}mper, {L H} and M Ernst and Flad, {H D} and J Gerdes",
year = "1995",
month = may,
doi = "10.1006/cyto.1995.0044",
language = "English",
volume = "7",
pages = "344--54",
journal = "Cytokine",
issn = "1043-4666",
publisher = "Elsevier",
number = "4",

}

RIS

TY - JOUR

T1 - The human germinal centre cells, follicular dendritic cells and germinal centre T cells produce B cell-stimulating cytokines

AU - Toellner, K M

AU - Scheel-Toellner, D

AU - Sprenger, R

AU - Duchrow, M

AU - Trümper, L H

AU - Ernst, M

AU - Flad, H D

AU - Gerdes, J

PY - 1995/5

Y1 - 1995/5

N2 - Cytokine gene expression was investigated in the germinal centre constituents of follicular dendritic cells and germinal centre T cells and compared to the mRNA expression of nongerminal centre tonsillar cells. Cells were isolated from human tonsils by preenrichment with MACS and subsequent FACS sorting. Cytokine gene expression was investigated by intronspanning RT-PCR for IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-10, IFN-alpha, IFN-beta, IFN-gamma, and TNF-alpha. Frequency of cytokine-producing cells and the cytokine production pattern of single cells were determined by single cell PCR. Furthermore, cytokine protein expression was investigated by immunohistology. Using these methods, we found a strong production of IL-1 beta mRNA and protein in a small percentage of FDC. Germinal centre T cells showed production of IL-2, IL-4, IL-10, IFN-gamma, and TNF-alpha mRNA. The mRNAs of these cytokines could also be detected at the single cell level; as they were produced in a high percentage of germinal centre T cells, whereas immunohistological staining was negative, we conclude that a high percentage of germinal centre T lymphocytes produce IL-2, IL-4, IL-10, IFN-gamma, and TNF-alpha in low concentrations in contrast to T cells outside the germinal centre, in which strong cytokine production in few cells was shown earlier.

AB - Cytokine gene expression was investigated in the germinal centre constituents of follicular dendritic cells and germinal centre T cells and compared to the mRNA expression of nongerminal centre tonsillar cells. Cells were isolated from human tonsils by preenrichment with MACS and subsequent FACS sorting. Cytokine gene expression was investigated by intronspanning RT-PCR for IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-10, IFN-alpha, IFN-beta, IFN-gamma, and TNF-alpha. Frequency of cytokine-producing cells and the cytokine production pattern of single cells were determined by single cell PCR. Furthermore, cytokine protein expression was investigated by immunohistology. Using these methods, we found a strong production of IL-1 beta mRNA and protein in a small percentage of FDC. Germinal centre T cells showed production of IL-2, IL-4, IL-10, IFN-gamma, and TNF-alpha mRNA. The mRNAs of these cytokines could also be detected at the single cell level; as they were produced in a high percentage of germinal centre T cells, whereas immunohistological staining was negative, we conclude that a high percentage of germinal centre T lymphocytes produce IL-2, IL-4, IL-10, IFN-gamma, and TNF-alpha in low concentrations in contrast to T cells outside the germinal centre, in which strong cytokine production in few cells was shown earlier.

KW - Dendritic Cells

KW - Tumor Necrosis Factor-alpha

KW - Humans

KW - Interferons

KW - Interleukins

KW - Child

KW - Cell Separation

KW - B-Lymphocytes

KW - RNA, Messenger

KW - Polymerase Chain Reaction

KW - Base Sequence

KW - Palatine Tonsil

KW - Molecular Sequence Data

KW - Cytokines

KW - Flow Cytometry

KW - Gene Expression Regulation

KW - Immunoenzyme Techniques

KW - T-Lymphocytes

U2 - 10.1006/cyto.1995.0044

DO - 10.1006/cyto.1995.0044

M3 - Article

C2 - 8589265

VL - 7

SP - 344

EP - 354

JO - Cytokine

JF - Cytokine

SN - 1043-4666

IS - 4

ER -