The human germinal centre cells, follicular dendritic cells and germinal centre T cells produce B cell-stimulating cytokines

Research output: Contribution to journalArticlepeer-review

Authors

  • R Sprenger
  • M Duchrow
  • L H Trümper
  • M Ernst
  • H D Flad
  • J Gerdes

Colleges, School and Institutes

Abstract

Cytokine gene expression was investigated in the germinal centre constituents of follicular dendritic cells and germinal centre T cells and compared to the mRNA expression of nongerminal centre tonsillar cells. Cells were isolated from human tonsils by preenrichment with MACS and subsequent FACS sorting. Cytokine gene expression was investigated by intronspanning RT-PCR for IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-10, IFN-alpha, IFN-beta, IFN-gamma, and TNF-alpha. Frequency of cytokine-producing cells and the cytokine production pattern of single cells were determined by single cell PCR. Furthermore, cytokine protein expression was investigated by immunohistology. Using these methods, we found a strong production of IL-1 beta mRNA and protein in a small percentage of FDC. Germinal centre T cells showed production of IL-2, IL-4, IL-10, IFN-gamma, and TNF-alpha mRNA. The mRNAs of these cytokines could also be detected at the single cell level; as they were produced in a high percentage of germinal centre T cells, whereas immunohistological staining was negative, we conclude that a high percentage of germinal centre T lymphocytes produce IL-2, IL-4, IL-10, IFN-gamma, and TNF-alpha in low concentrations in contrast to T cells outside the germinal centre, in which strong cytokine production in few cells was shown earlier.

Details

Original languageEnglish
Pages (from-to)344-54
Number of pages11
JournalCytokine
Volume7
Issue number4
Publication statusPublished - May 1995

Keywords

  • Dendritic Cells, Tumor Necrosis Factor-alpha, Humans, Interferons, Interleukins, Child, Cell Separation, B-Lymphocytes, RNA, Messenger, Polymerase Chain Reaction, Base Sequence, Palatine Tonsil, Molecular Sequence Data, Cytokines, Flow Cytometry, Gene Expression Regulation, Immunoenzyme Techniques, T-Lymphocytes