The functional subunit of a dimeric transcription activator protein depends on promoter architecture

Research output: Contribution to journalArticlepeer-review

Authors

  • Y Zhou
  • P S Pendergrast
  • R Williams
  • R H Ebright

Colleges, School and Institutes

Abstract

In Class I CAP-dependent promoters, the DNA site for CAP is located upstream of the DNA site for RNA polymerase. In Class II CAP-dependent promoters, the DNA site for CAP overlaps the DNA site for RNA polymerase, replacing the -35 site. We have used an 'oriented heterodimers' approach to identify the functional subunit of CAP at two Class I promoters having different distances between the DNA sites for CAP and RNA polymerase [CC(-61.5) and CC(-72.5)] and at one Class II promoter [CC(-41.5)]. Our results indicate that transcription activation at Class I promoters, irrespective of the distance between the DNA sites for CAP and RNA polymerase, requires the activating region of the promoter-proximal subunit of CAP. In striking contrast, our results indicate that transcription activation at Class II promoters requires the activating region of the promoter-distal subunit of CAP.

Details

Original languageEnglish
Pages (from-to)4549-57
Number of pages9
JournalThe EMBO journal
Volume13
Issue number19
Publication statusPublished - 1994