The Fc receptor gamma-chain is necessary and sufficient to initiate signalling through glycoprotein VI

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The Fc receptor gamma-chain is necessary and sufficient to initiate signalling through glycoprotein VI. / Berlanga, Oscar; Tulasne, D; Snell, DC; Jung, S; Moroi, M; Frampton, Jonathan; Watson, SP.

In: European Journal of Biochemistry, Vol. 269, No. 12, 01.06.2002, p. 2951-2960.

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@article{e60bbb5b9b5a48ab93363b8ccda42584,
title = "The Fc receptor gamma-chain is necessary and sufficient to initiate signalling through glycoprotein VI",
abstract = "There is extensive evidence that FcR gamma-chain couples to the collagen receptor glycoprotein VI (GPVI) and becomes phosphorylated on tyrosines upon receptor cross-linking. However, it is not established whether this receptor complex is sufficient to initiate the signalling cascade. We transfected GPVI and the FcR gamma-chain into the human erythroleukaemia cell line K562, which lacks detectable expression of GPVI and the FcR gamma-chain. The results show that GPVI is unable to signal when expressed alone, despite its surface expression, upon stimulation with the snake C-type lectin, convulxin. Coexpression of the FcR gamma-chain confers signalling properties on the receptor. Furthermore, cotransfection of the FcR gamma-chain and two mutant versions of GPVI shows that the transmembrane arginine and cytoplasmic tail of GPVI are necessary for association with the FcR gamma-chain. These results demonstrate that reconstitution of the GPVI-FcR gamma-chain complex in cells expressing the necessary signalling network is sufficient to initiate signalling events in response to convulxin and collagen-related peptide.",
keywords = "convulxin, collagen, collagen-related peptide (CRP), glycoprotein VI (GPVI), FcR gamma-chain",
author = "Oscar Berlanga and D Tulasne and DC Snell and S Jung and M Moroi and Jonathan Frampton and SP Watson",
year = "2002",
month = jun,
day = "1",
doi = "10.1046/j.1432-1033.2002.02969.x",
language = "English",
volume = "269",
pages = "2951--2960",
journal = "European Journal of Biochemistry",
issn = "0014-2956",
publisher = "Wiley",
number = "12",

}

RIS

TY - JOUR

T1 - The Fc receptor gamma-chain is necessary and sufficient to initiate signalling through glycoprotein VI

AU - Berlanga, Oscar

AU - Tulasne, D

AU - Snell, DC

AU - Jung, S

AU - Moroi, M

AU - Frampton, Jonathan

AU - Watson, SP

PY - 2002/6/1

Y1 - 2002/6/1

N2 - There is extensive evidence that FcR gamma-chain couples to the collagen receptor glycoprotein VI (GPVI) and becomes phosphorylated on tyrosines upon receptor cross-linking. However, it is not established whether this receptor complex is sufficient to initiate the signalling cascade. We transfected GPVI and the FcR gamma-chain into the human erythroleukaemia cell line K562, which lacks detectable expression of GPVI and the FcR gamma-chain. The results show that GPVI is unable to signal when expressed alone, despite its surface expression, upon stimulation with the snake C-type lectin, convulxin. Coexpression of the FcR gamma-chain confers signalling properties on the receptor. Furthermore, cotransfection of the FcR gamma-chain and two mutant versions of GPVI shows that the transmembrane arginine and cytoplasmic tail of GPVI are necessary for association with the FcR gamma-chain. These results demonstrate that reconstitution of the GPVI-FcR gamma-chain complex in cells expressing the necessary signalling network is sufficient to initiate signalling events in response to convulxin and collagen-related peptide.

AB - There is extensive evidence that FcR gamma-chain couples to the collagen receptor glycoprotein VI (GPVI) and becomes phosphorylated on tyrosines upon receptor cross-linking. However, it is not established whether this receptor complex is sufficient to initiate the signalling cascade. We transfected GPVI and the FcR gamma-chain into the human erythroleukaemia cell line K562, which lacks detectable expression of GPVI and the FcR gamma-chain. The results show that GPVI is unable to signal when expressed alone, despite its surface expression, upon stimulation with the snake C-type lectin, convulxin. Coexpression of the FcR gamma-chain confers signalling properties on the receptor. Furthermore, cotransfection of the FcR gamma-chain and two mutant versions of GPVI shows that the transmembrane arginine and cytoplasmic tail of GPVI are necessary for association with the FcR gamma-chain. These results demonstrate that reconstitution of the GPVI-FcR gamma-chain complex in cells expressing the necessary signalling network is sufficient to initiate signalling events in response to convulxin and collagen-related peptide.

KW - convulxin

KW - collagen

KW - collagen-related peptide (CRP)

KW - glycoprotein VI (GPVI)

KW - FcR gamma-chain

UR - http://www.scopus.com/inward/record.url?scp=0036318095&partnerID=8YFLogxK

U2 - 10.1046/j.1432-1033.2002.02969.x

DO - 10.1046/j.1432-1033.2002.02969.x

M3 - Article

C2 - 12071959

VL - 269

SP - 2951

EP - 2960

JO - European Journal of Biochemistry

JF - European Journal of Biochemistry

SN - 0014-2956

IS - 12

ER -