The Fc receptor gamma-chain is necessary and sufficient to initiate signalling through glycoprotein VI
Research output: Contribution to journal › Article
Authors
Colleges, School and Institutes
Abstract
There is extensive evidence that FcR gamma-chain couples to the collagen receptor glycoprotein VI (GPVI) and becomes phosphorylated on tyrosines upon receptor cross-linking. However, it is not established whether this receptor complex is sufficient to initiate the signalling cascade. We transfected GPVI and the FcR gamma-chain into the human erythroleukaemia cell line K562, which lacks detectable expression of GPVI and the FcR gamma-chain. The results show that GPVI is unable to signal when expressed alone, despite its surface expression, upon stimulation with the snake C-type lectin, convulxin. Coexpression of the FcR gamma-chain confers signalling properties on the receptor. Furthermore, cotransfection of the FcR gamma-chain and two mutant versions of GPVI shows that the transmembrane arginine and cytoplasmic tail of GPVI are necessary for association with the FcR gamma-chain. These results demonstrate that reconstitution of the GPVI-FcR gamma-chain complex in cells expressing the necessary signalling network is sufficient to initiate signalling events in response to convulxin and collagen-related peptide.
Details
Original language | English |
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Pages (from-to) | 2951-2960 |
Number of pages | 10 |
Journal | European Journal of Biochemistry |
Volume | 269 |
Issue number | 12 |
Publication status | Published - 1 Jun 2002 |
Keywords
- convulxin, collagen, collagen-related peptide (CRP), glycoprotein VI (GPVI), FcR gamma-chain