The Fc receptor gamma-chain is necessary and sufficient to initiate signalling through glycoprotein VI

Research output: Contribution to journalArticle

Authors

  • D Tulasne
  • DC Snell
  • S Jung
  • M Moroi
  • SP Watson

Abstract

There is extensive evidence that FcR gamma-chain couples to the collagen receptor glycoprotein VI (GPVI) and becomes phosphorylated on tyrosines upon receptor cross-linking. However, it is not established whether this receptor complex is sufficient to initiate the signalling cascade. We transfected GPVI and the FcR gamma-chain into the human erythroleukaemia cell line K562, which lacks detectable expression of GPVI and the FcR gamma-chain. The results show that GPVI is unable to signal when expressed alone, despite its surface expression, upon stimulation with the snake C-type lectin, convulxin. Coexpression of the FcR gamma-chain confers signalling properties on the receptor. Furthermore, cotransfection of the FcR gamma-chain and two mutant versions of GPVI shows that the transmembrane arginine and cytoplasmic tail of GPVI are necessary for association with the FcR gamma-chain. These results demonstrate that reconstitution of the GPVI-FcR gamma-chain complex in cells expressing the necessary signalling network is sufficient to initiate signalling events in response to convulxin and collagen-related peptide.

Details

Original languageEnglish
Pages (from-to)2951-2960
Number of pages10
JournalEuropean Journal of Biochemistry
Volume269
Issue number12
Publication statusPublished - 1 Jun 2002

Keywords

  • convulxin, collagen, collagen-related peptide (CRP), glycoprotein VI (GPVI), FcR gamma-chain