TY - JOUR
T1 - The expression profile of Escherichia coli K-12 in response to minimal, optimal and excess copper concentrations
AU - Kershaw, Christopher
AU - Brown, Nigel
AU - Constantinidou, Chrystala
AU - Patel, Mala
AU - Hobman, Jonathan
PY - 2005/4/1
Y1 - 2005/4/1
N2 - The gene expression profile of Escherichia coli K-12 MG1655 grown in minimal medium supplemented with elevated copper concentrations (as copper-glycine) has been analysed using whole-genome oligonucleotide microarrays. At 750 muM copper-glycine, the expression of both the cue and cus copper-export systems is evident. At near-lethal copper concentrations (2 mM copper-glycine), the expression of these two regulons increases significantly. Other regulons with increased transcription in response to elevated concentrations of copper-glycine include those for the superoxide stress response, iron homeostasis, and envelope stress. Furthermore, a variety of ORFs with decreased expression in response to increased copper-glycine has been identified, including the zinc ABC transporter and genes involved in the chemotactic response.
AB - The gene expression profile of Escherichia coli K-12 MG1655 grown in minimal medium supplemented with elevated copper concentrations (as copper-glycine) has been analysed using whole-genome oligonucleotide microarrays. At 750 muM copper-glycine, the expression of both the cue and cus copper-export systems is evident. At near-lethal copper concentrations (2 mM copper-glycine), the expression of these two regulons increases significantly. Other regulons with increased transcription in response to elevated concentrations of copper-glycine include those for the superoxide stress response, iron homeostasis, and envelope stress. Furthermore, a variety of ORFs with decreased expression in response to increased copper-glycine has been identified, including the zinc ABC transporter and genes involved in the chemotactic response.
UR - http://www.scopus.com/inward/record.url?scp=17644374089&partnerID=8YFLogxK
U2 - 10.1099/mic.0.27650-0
DO - 10.1099/mic.0.27650-0
M3 - Article
C2 - 15817786
SN - 1465-2080
VL - 151
SP - 1187
EP - 1198
JO - Microbiology
JF - Microbiology
ER -