The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state

Research output: Contribution to journalArticle

External organisations

  • MRC Centre for Immune Regulation, Division of Immunity and Infection, Birmingham University, Birmingham, UK.
  • Centre for Translational Inflammation Research; Rheumatology Research Group; University of Birmingham; Birmingham UK
  • Medical School; University of Oxford; Oxford UK
  • Department of Experimental Biomedicine; University Hospital, University of Würzburg; Würzburg Germany

Abstract

Expression of mouse C-type lectin-like receptor 2 (CLEC-2) has been reported on circulating CD11bhigh Gr-1high myeloid cells and dendritic cells (DCs) under basal conditions, as well as on a variety of leucocyte subsets following inflammatory stimuli or in vitro cell culture. However, previous studies assessing CLEC-2 expression failed to use CLEC-2-deficient mice as negative controls and instead relied heavily on single antibody clones. Here, we generated CLEC-2-deficient adult mice using two independent approaches and employed two anti-mouse CLEC-2 antibody clones to investigate surface expression on hematopoietic cells from peripheral blood and secondary lymphoid organs. We rule out constitutive CLEC-2 expression on resting DCs and show that CLEC-2 is upregulated in response to LPS-induced systemic inflammation in a small subset of activated DCs isolated from the mesenteric lymph nodes but not the spleen. Moreover, we demonstrate for the first time that peripheral blood B lymphocytes present exogenously derived CLEC-2 and suggest that both circulating B lymphocytes and CD11bhigh Gr-1high myeloid cells lose CLEC-2 following entry into secondary lymphoid organs. These results have significant implications for our understanding of CLEC-2 physiological functions

Details

Original languageEnglish
Pages (from-to)2484–2493
JournalEuropean Journal of Immunology
Volume45
Issue number9
Early online date12 Aug 2015
Publication statusPublished - 7 Sep 2015

Keywords

  • CLEC-2, Inflammation, Leucocytes, Mouse, Tamoxifen