The Escherichia coli Regulator of Sigma 70 Protein, Rsd, Can Up-Regulate Some Stress-Dependent Promoters by Sequestering Sigma 70

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The Escherichia coli Regulator of Sigma 70 Protein, Rsd, Can Up-Regulate Some Stress-Dependent Promoters by Sequestering Sigma 70. / Mitchell, Jennie; Oshima, T; Piper, Sarah; Webster, Christine; Westblade, LF; Karimova, G; Ladant, D; Kolb, A; Hobman, Jonathan; Busby, Stephen; Lee, David.

In: Journal of Bacteriology, Vol. 189, No. 9, 02.03.2007, p. 3489-3495.

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Mitchell, Jennie ; Oshima, T ; Piper, Sarah ; Webster, Christine ; Westblade, LF ; Karimova, G ; Ladant, D ; Kolb, A ; Hobman, Jonathan ; Busby, Stephen ; Lee, David. / The Escherichia coli Regulator of Sigma 70 Protein, Rsd, Can Up-Regulate Some Stress-Dependent Promoters by Sequestering Sigma 70. In: Journal of Bacteriology. 2007 ; Vol. 189, No. 9. pp. 3489-3495.

Bibtex

@article{d4ab91d94b04414eb01df3b92336c8d0,
title = "The Escherichia coli Regulator of Sigma 70 Protein, Rsd, Can Up-Regulate Some Stress-Dependent Promoters by Sequestering Sigma 70",
abstract = "The Escherichia coli Rsd protein forms complexes with the RNA polymerase sigma(70) factor, but its biological role is not understood. Transcriptome analysis shows that overexpression of Rsd causes increased expression from some promoters whose expression depends on the alternative sigma(38) factor, and this was confirmed by experiments with lac fusions at selected promoters. The LP18 substitution in Rsd increases the Rsd-dependent stimulation of these promoter-lac fusions. Analysis with a bacterial two-hybrid system shows that the LP18 substitution in Rsd increases its interaction with sigma(70). Our experiments support a model in which the role of Rsd is primarily to sequester sigma(70), thereby increasing the levels of RNA polymerase containing the alternative sigma(38) factor.",
author = "Jennie Mitchell and T Oshima and Sarah Piper and Christine Webster and LF Westblade and G Karimova and D Ladant and A Kolb and Jonathan Hobman and Stephen Busby and David Lee",
year = "2007",
month = mar,
day = "2",
doi = "10.1128/JB.00019-07",
language = "English",
volume = "189",
pages = "3489--3495",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "9",

}

RIS

TY - JOUR

T1 - The Escherichia coli Regulator of Sigma 70 Protein, Rsd, Can Up-Regulate Some Stress-Dependent Promoters by Sequestering Sigma 70

AU - Mitchell, Jennie

AU - Oshima, T

AU - Piper, Sarah

AU - Webster, Christine

AU - Westblade, LF

AU - Karimova, G

AU - Ladant, D

AU - Kolb, A

AU - Hobman, Jonathan

AU - Busby, Stephen

AU - Lee, David

PY - 2007/3/2

Y1 - 2007/3/2

N2 - The Escherichia coli Rsd protein forms complexes with the RNA polymerase sigma(70) factor, but its biological role is not understood. Transcriptome analysis shows that overexpression of Rsd causes increased expression from some promoters whose expression depends on the alternative sigma(38) factor, and this was confirmed by experiments with lac fusions at selected promoters. The LP18 substitution in Rsd increases the Rsd-dependent stimulation of these promoter-lac fusions. Analysis with a bacterial two-hybrid system shows that the LP18 substitution in Rsd increases its interaction with sigma(70). Our experiments support a model in which the role of Rsd is primarily to sequester sigma(70), thereby increasing the levels of RNA polymerase containing the alternative sigma(38) factor.

AB - The Escherichia coli Rsd protein forms complexes with the RNA polymerase sigma(70) factor, but its biological role is not understood. Transcriptome analysis shows that overexpression of Rsd causes increased expression from some promoters whose expression depends on the alternative sigma(38) factor, and this was confirmed by experiments with lac fusions at selected promoters. The LP18 substitution in Rsd increases the Rsd-dependent stimulation of these promoter-lac fusions. Analysis with a bacterial two-hybrid system shows that the LP18 substitution in Rsd increases its interaction with sigma(70). Our experiments support a model in which the role of Rsd is primarily to sequester sigma(70), thereby increasing the levels of RNA polymerase containing the alternative sigma(38) factor.

U2 - 10.1128/JB.00019-07

DO - 10.1128/JB.00019-07

M3 - Article

C2 - 17351046

VL - 189

SP - 3489

EP - 3495

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 9

ER -