The Escherichia coli MarA protein regulates the ycgZ-ymgABC operon to inhibit biofilm formation

Research output: Contribution to journalArticle

Standard

The Escherichia coli MarA protein regulates the ycgZ-ymgABC operon to inhibit biofilm formation. / Kettles, Rachel A; Tschowri, Natalia; Lyons, Kevin J; Sharma, Prateek; Hengge, Regine; Webber, Mark A; Grainger, David C.

In: Molecular Microbiology, Vol. 112, No. 5, 11.2019, p. 1609-1625.

Research output: Contribution to journalArticle

Harvard

APA

Vancouver

Author

Kettles, Rachel A ; Tschowri, Natalia ; Lyons, Kevin J ; Sharma, Prateek ; Hengge, Regine ; Webber, Mark A ; Grainger, David C. / The Escherichia coli MarA protein regulates the ycgZ-ymgABC operon to inhibit biofilm formation. In: Molecular Microbiology. 2019 ; Vol. 112, No. 5. pp. 1609-1625.

Bibtex

@article{1150263b75a64e9fafbe165bbd7e2259,
title = "The Escherichia coli MarA protein regulates the ycgZ-ymgABC operon to inhibit biofilm formation",
abstract = "The Escherichia coli marRAB operon is a paradigm for chromosomally encoded antibiotic resistance. The operon exerts its effect via an encoded transcription factor called MarA that modulates efflux pump and porin expression. In this work, we show that MarA is also a regulator of biofilm formation. Control is mediated by binding of MarA to the intergenic region upstream of the ycgZ-ymgABC operon. The operon, known to influence the formation of curli fibres and colanic acid, is usually expressed during periods of starvation. Hence, the ycgZ-ymgABC promoter is recognised by σ38 (RpoS)-associated RNA polymerase (RNAP). Surprisingly, MarA does not influence σ38 -dependent transcription. Instead, MarA drives transcription by the housekeeping σ70 -associated RNAP. The effects of MarA on ycgZ-ymgABC expression are coupled with biofilm formation by the rcsCDB phosphorelay system, with YcgZ, YmgA and YmgB forming a complex that directly interacts with the histidine kinase domain of RcsC.",
author = "Kettles, {Rachel A} and Natalia Tschowri and Lyons, {Kevin J} and Prateek Sharma and Regine Hengge and Webber, {Mark A} and Grainger, {David C}",
note = "{\textcopyright} 2019 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.",
year = "2019",
month = nov,
doi = "10.1111/mmi.14386",
language = "English",
volume = "112",
pages = "1609--1625",
journal = "Molecular Microbiology",
issn = "0950-382X",
publisher = "Wiley",
number = "5",

}

RIS

TY - JOUR

T1 - The Escherichia coli MarA protein regulates the ycgZ-ymgABC operon to inhibit biofilm formation

AU - Kettles, Rachel A

AU - Tschowri, Natalia

AU - Lyons, Kevin J

AU - Sharma, Prateek

AU - Hengge, Regine

AU - Webber, Mark A

AU - Grainger, David C

N1 - © 2019 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.

PY - 2019/11

Y1 - 2019/11

N2 - The Escherichia coli marRAB operon is a paradigm for chromosomally encoded antibiotic resistance. The operon exerts its effect via an encoded transcription factor called MarA that modulates efflux pump and porin expression. In this work, we show that MarA is also a regulator of biofilm formation. Control is mediated by binding of MarA to the intergenic region upstream of the ycgZ-ymgABC operon. The operon, known to influence the formation of curli fibres and colanic acid, is usually expressed during periods of starvation. Hence, the ycgZ-ymgABC promoter is recognised by σ38 (RpoS)-associated RNA polymerase (RNAP). Surprisingly, MarA does not influence σ38 -dependent transcription. Instead, MarA drives transcription by the housekeeping σ70 -associated RNAP. The effects of MarA on ycgZ-ymgABC expression are coupled with biofilm formation by the rcsCDB phosphorelay system, with YcgZ, YmgA and YmgB forming a complex that directly interacts with the histidine kinase domain of RcsC.

AB - The Escherichia coli marRAB operon is a paradigm for chromosomally encoded antibiotic resistance. The operon exerts its effect via an encoded transcription factor called MarA that modulates efflux pump and porin expression. In this work, we show that MarA is also a regulator of biofilm formation. Control is mediated by binding of MarA to the intergenic region upstream of the ycgZ-ymgABC operon. The operon, known to influence the formation of curli fibres and colanic acid, is usually expressed during periods of starvation. Hence, the ycgZ-ymgABC promoter is recognised by σ38 (RpoS)-associated RNA polymerase (RNAP). Surprisingly, MarA does not influence σ38 -dependent transcription. Instead, MarA drives transcription by the housekeeping σ70 -associated RNAP. The effects of MarA on ycgZ-ymgABC expression are coupled with biofilm formation by the rcsCDB phosphorelay system, with YcgZ, YmgA and YmgB forming a complex that directly interacts with the histidine kinase domain of RcsC.

UR - http://www.scopus.com/inward/record.url?scp=85073931774&partnerID=8YFLogxK

U2 - 10.1111/mmi.14386

DO - 10.1111/mmi.14386

M3 - Article

C2 - 31518447

VL - 112

SP - 1609

EP - 1625

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 5

ER -