The effect of UV-Vis to near-infrared light on the biological response of human dental pulp cells

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Standard

The effect of UV-Vis to near-infrared light on the biological response of human dental pulp cells. / Hadis, Mohammed A.; Cooper, Paul R.; Milward, Michael R.; Gorecki, Patricia; Tarte, Edward; Churm, James; Palin, William M.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9309 Society of Photo-Optical Instrumentation Engineers, 2015. 930906.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Harvard

Hadis, MA, Cooper, PR, Milward, MR, Gorecki, P, Tarte, E, Churm, J & Palin, WM 2015, The effect of UV-Vis to near-infrared light on the biological response of human dental pulp cells. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 9309, 930906, Society of Photo-Optical Instrumentation Engineers, Mechanisms for Low-Light Therapy X, San Francisco, United States, 7/02/15. https://doi.org/10.1117/12.2077645

APA

Hadis, M. A., Cooper, P. R., Milward, M. R., Gorecki, P., Tarte, E., Churm, J., & Palin, W. M. (2015). The effect of UV-Vis to near-infrared light on the biological response of human dental pulp cells. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 9309). [930906] Society of Photo-Optical Instrumentation Engineers. https://doi.org/10.1117/12.2077645

Vancouver

Hadis MA, Cooper PR, Milward MR, Gorecki P, Tarte E, Churm J et al. The effect of UV-Vis to near-infrared light on the biological response of human dental pulp cells. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9309. Society of Photo-Optical Instrumentation Engineers. 2015. 930906 https://doi.org/10.1117/12.2077645

Author

Hadis, Mohammed A. ; Cooper, Paul R. ; Milward, Michael R. ; Gorecki, Patricia ; Tarte, Edward ; Churm, James ; Palin, William M. / The effect of UV-Vis to near-infrared light on the biological response of human dental pulp cells. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9309 Society of Photo-Optical Instrumentation Engineers, 2015.

Bibtex

@inproceedings{f6c1a4fa51314413b29405c464f6f500,
title = "The effect of UV-Vis to near-infrared light on the biological response of human dental pulp cells",
abstract = "Human dental pulp cells (DPCs) were isolated and cultured in phenol-red-free α-MEM/10%-FCS at 37°C in 5% CO2. DPCs at passages 2-4 were seeded (150μ1/4L; 25,000 cell/ml) in black 96-microwell plates with transparent bases. 24h post-seeding, cultures were irradiated using a bespoke LED array consisting of 60 LEDs (3.5mW/cm2) of wavelengths from 400-900nm (10 wavelengths, n=6) for time intervals of up to 120s. Metabolic and mitochondrial activity was assessed via a modified MTT assay. Statistical differences were identified using multi-factorial analysis of variance and post-hoc Tukey tests (P=0.05). The biological responses were significantly dependent upon post-irradiation incubation period, wavelength and exposure time (P<0.05). At shorter wavelength irradiances (400nm), a reduction in mitochondrial activity was detected although not significant, whereas longer wavelength irradiances (at 633, 656, 781 and 799nm) significantly increased mitochondrial activity (P<0.05) in DPCs. At these wavelengths, mitochondrial activity was generally increased for exposures less than 90s with 30s exposures being most effective with 24h incubation. Increasing the post-irradiation incubation period increased the measured response and identified further significance (P<0.05). The biological responses of human DPCs were wavelength, exposure-time and incubation period dependent. The optimisation of irradiation parameters will be key to the successful application of LLLT in dentistry.",
keywords = "dose, LED, LLLT, MTT, photobiomodulation, wavelength",
author = "Hadis, {Mohammed A.} and Cooper, {Paul R.} and Milward, {Michael R.} and Patricia Gorecki and Edward Tarte and James Churm and Palin, {William M.}",
year = "2015",
doi = "10.1117/12.2077645",
language = "English",
isbn = "9781628413991",
volume = "9309",
booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "Society of Photo-Optical Instrumentation Engineers",
address = "United States",
note = "Mechanisms for Low-Light Therapy X ; Conference date: 07-02-2015 Through 08-02-2015",

}

RIS

TY - GEN

T1 - The effect of UV-Vis to near-infrared light on the biological response of human dental pulp cells

AU - Hadis, Mohammed A.

AU - Cooper, Paul R.

AU - Milward, Michael R.

AU - Gorecki, Patricia

AU - Tarte, Edward

AU - Churm, James

AU - Palin, William M.

PY - 2015

Y1 - 2015

N2 - Human dental pulp cells (DPCs) were isolated and cultured in phenol-red-free α-MEM/10%-FCS at 37°C in 5% CO2. DPCs at passages 2-4 were seeded (150μ1/4L; 25,000 cell/ml) in black 96-microwell plates with transparent bases. 24h post-seeding, cultures were irradiated using a bespoke LED array consisting of 60 LEDs (3.5mW/cm2) of wavelengths from 400-900nm (10 wavelengths, n=6) for time intervals of up to 120s. Metabolic and mitochondrial activity was assessed via a modified MTT assay. Statistical differences were identified using multi-factorial analysis of variance and post-hoc Tukey tests (P=0.05). The biological responses were significantly dependent upon post-irradiation incubation period, wavelength and exposure time (P<0.05). At shorter wavelength irradiances (400nm), a reduction in mitochondrial activity was detected although not significant, whereas longer wavelength irradiances (at 633, 656, 781 and 799nm) significantly increased mitochondrial activity (P<0.05) in DPCs. At these wavelengths, mitochondrial activity was generally increased for exposures less than 90s with 30s exposures being most effective with 24h incubation. Increasing the post-irradiation incubation period increased the measured response and identified further significance (P<0.05). The biological responses of human DPCs were wavelength, exposure-time and incubation period dependent. The optimisation of irradiation parameters will be key to the successful application of LLLT in dentistry.

AB - Human dental pulp cells (DPCs) were isolated and cultured in phenol-red-free α-MEM/10%-FCS at 37°C in 5% CO2. DPCs at passages 2-4 were seeded (150μ1/4L; 25,000 cell/ml) in black 96-microwell plates with transparent bases. 24h post-seeding, cultures were irradiated using a bespoke LED array consisting of 60 LEDs (3.5mW/cm2) of wavelengths from 400-900nm (10 wavelengths, n=6) for time intervals of up to 120s. Metabolic and mitochondrial activity was assessed via a modified MTT assay. Statistical differences were identified using multi-factorial analysis of variance and post-hoc Tukey tests (P=0.05). The biological responses were significantly dependent upon post-irradiation incubation period, wavelength and exposure time (P<0.05). At shorter wavelength irradiances (400nm), a reduction in mitochondrial activity was detected although not significant, whereas longer wavelength irradiances (at 633, 656, 781 and 799nm) significantly increased mitochondrial activity (P<0.05) in DPCs. At these wavelengths, mitochondrial activity was generally increased for exposures less than 90s with 30s exposures being most effective with 24h incubation. Increasing the post-irradiation incubation period increased the measured response and identified further significance (P<0.05). The biological responses of human DPCs were wavelength, exposure-time and incubation period dependent. The optimisation of irradiation parameters will be key to the successful application of LLLT in dentistry.

KW - dose

KW - LED

KW - LLLT

KW - MTT

KW - photobiomodulation

KW - wavelength

UR - http://www.scopus.com/inward/record.url?scp=84926430003&partnerID=8YFLogxK

U2 - 10.1117/12.2077645

DO - 10.1117/12.2077645

M3 - Conference contribution

AN - SCOPUS:84926430003

SN - 9781628413991

VL - 9309

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

PB - Society of Photo-Optical Instrumentation Engineers

T2 - Mechanisms for Low-Light Therapy X

Y2 - 7 February 2015 through 8 February 2015

ER -