The effect of type 1 IFN on human aortic endothelial cell function in vitro: Relevance to systemic lupus erythematosus

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The effect of type 1 IFN on human aortic endothelial cell function in vitro : Relevance to systemic lupus erythematosus. / Reynolds, John A.; Ray, David W.; Zeef, Leo A.H.; O'Neill, Terence; Bruce, Ian N.; Alexander, M. Yvonne.

In: Journal of Interferon and Cytokine Research, Vol. 34, No. 5, 01.05.2014, p. 404-412.

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Reynolds, John A. ; Ray, David W. ; Zeef, Leo A.H. ; O'Neill, Terence ; Bruce, Ian N. ; Alexander, M. Yvonne. / The effect of type 1 IFN on human aortic endothelial cell function in vitro : Relevance to systemic lupus erythematosus. In: Journal of Interferon and Cytokine Research. 2014 ; Vol. 34, No. 5. pp. 404-412.

Bibtex

@article{6c8c080a06c740ff85b3dad3a7583826,
title = "The effect of type 1 IFN on human aortic endothelial cell function in vitro: Relevance to systemic lupus erythematosus",
abstract = "Cardiovascular disease (CVD) is an important cause of morbidity and mortality in patients with systemic lupus erythematosus. The etiopathogenesis of premature CVD is not fully understood, but recently interferon-alpha (IFNα) has been implicated as a contributing factor. Since IFNα has been associated with both disease activity and endothelial dysfunction in lupus patients, we aimed to determine whether IFNα has direct effects on human aortic endothelial cell (HAoEC) function in vitro. We studied the function of IFNα2b-treated HAoECs in terms of cell proliferation, capillary-like network formation, and nitric oxide (NO) generation. Changes in gene expression were also analyzed using an exon gene array. IFNα2b regulated the expression of 198 genes, including recognized interferon-stimulated genes (ISGs). Gene ontology analysis showed over-representation of genes involved in antigen presentation and host response to virus but no significant changes in clusters of genes recognized as important in endothelial cell activation or dysfunction. HAoEC proliferation, tubule formation, and NO bioavailability were unchanged, suggesting that IFNα in isolation does not have a direct impact on aortic endothelial cell function.",
author = "Reynolds, {John A.} and Ray, {David W.} and Zeef, {Leo A.H.} and Terence O'Neill and Bruce, {Ian N.} and Alexander, {M. Yvonne}",
year = "2014",
month = may,
day = "1",
doi = "10.1089/jir.2013.0016",
language = "English",
volume = "34",
pages = "404--412",
journal = "Journal of Interferon & Cytokine Research",
issn = "1079-9907",
publisher = "Mary Ann Liebert",
number = "5",

}

RIS

TY - JOUR

T1 - The effect of type 1 IFN on human aortic endothelial cell function in vitro

T2 - Relevance to systemic lupus erythematosus

AU - Reynolds, John A.

AU - Ray, David W.

AU - Zeef, Leo A.H.

AU - O'Neill, Terence

AU - Bruce, Ian N.

AU - Alexander, M. Yvonne

PY - 2014/5/1

Y1 - 2014/5/1

N2 - Cardiovascular disease (CVD) is an important cause of morbidity and mortality in patients with systemic lupus erythematosus. The etiopathogenesis of premature CVD is not fully understood, but recently interferon-alpha (IFNα) has been implicated as a contributing factor. Since IFNα has been associated with both disease activity and endothelial dysfunction in lupus patients, we aimed to determine whether IFNα has direct effects on human aortic endothelial cell (HAoEC) function in vitro. We studied the function of IFNα2b-treated HAoECs in terms of cell proliferation, capillary-like network formation, and nitric oxide (NO) generation. Changes in gene expression were also analyzed using an exon gene array. IFNα2b regulated the expression of 198 genes, including recognized interferon-stimulated genes (ISGs). Gene ontology analysis showed over-representation of genes involved in antigen presentation and host response to virus but no significant changes in clusters of genes recognized as important in endothelial cell activation or dysfunction. HAoEC proliferation, tubule formation, and NO bioavailability were unchanged, suggesting that IFNα in isolation does not have a direct impact on aortic endothelial cell function.

AB - Cardiovascular disease (CVD) is an important cause of morbidity and mortality in patients with systemic lupus erythematosus. The etiopathogenesis of premature CVD is not fully understood, but recently interferon-alpha (IFNα) has been implicated as a contributing factor. Since IFNα has been associated with both disease activity and endothelial dysfunction in lupus patients, we aimed to determine whether IFNα has direct effects on human aortic endothelial cell (HAoEC) function in vitro. We studied the function of IFNα2b-treated HAoECs in terms of cell proliferation, capillary-like network formation, and nitric oxide (NO) generation. Changes in gene expression were also analyzed using an exon gene array. IFNα2b regulated the expression of 198 genes, including recognized interferon-stimulated genes (ISGs). Gene ontology analysis showed over-representation of genes involved in antigen presentation and host response to virus but no significant changes in clusters of genes recognized as important in endothelial cell activation or dysfunction. HAoEC proliferation, tubule formation, and NO bioavailability were unchanged, suggesting that IFNα in isolation does not have a direct impact on aortic endothelial cell function.

UR - http://www.scopus.com/inward/record.url?scp=84900343979&partnerID=8YFLogxK

U2 - 10.1089/jir.2013.0016

DO - 10.1089/jir.2013.0016

M3 - Article

C2 - 24444308

AN - SCOPUS:84900343979

VL - 34

SP - 404

EP - 412

JO - Journal of Interferon & Cytokine Research

JF - Journal of Interferon & Cytokine Research

SN - 1079-9907

IS - 5

ER -