The effect of nutrient limitation on biofilm formation and phosphatase activity of a Citrobacter sp

Research output: Contribution to journalArticle

Colleges, School and Institutes

Abstract

A phosphatase-overproducing Citrobacter sp. (NCIMB 40259) was grown in an air-lift reactor in steady-state continuous culture under limitation of carbon, phosphorus or nitrogen. Substantial biofilm formation, and the highest phosphatase activity, were observed under lactose limitation. However, the total amount of biofilm wet biomass and the phosphatase specific activity were reduced in phosphorus- or nitrogen-limited cultures or when glucose was substituted for lactose as the limiting carbon source. Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM) showed differences in cell and biofilm morphology in relation to medium composition. Electron microscopy suggested that the differences in biofilm formation may relate to differential expression of fimbriae on the cell surface.

Details

Original languageEnglish
Pages (from-to)277-288
Number of pages12
JournalMicrobiology
Volume148
Publication statusPublished - 1 Jan 2002

Keywords

  • fimbriae, starvation, phosphohydrolase, confocal laser scanning microscopy, attached cells