The effect of antigen cross-linking on the sensitivity of the enzyme-linked immunosorbent assay

Research output: Contribution to journalArticle

Authors

Colleges, School and Institutes

Abstract

To investigate the effects of antigen cross-linking on ELISA results, we assayed antibodies to several purified antigens, using microtitration trays coated with antigens in their native and cross-linked forms. Cross-linking was achieved by a carbodiimide promoted condensation reaction. For several antigens an increase in sensitivity of the ELISA was obtained after cross-linking. The results of a double antibody sandwich assay using the same cross-linked antigens, showed that the affinity for antibodies remained unchanged after limited cross-linking. Therefore, the higher sensitivity of the ELISA is most likely due to a higher affinity of the cross-linked antigens for the plastic carrier. Cross-linking of proteins in a complex antigen preparation obtained from Schistosoma mansoni led to a large increase in sensitivity.

Details

Original languageEnglish
Pages (from-to)53-64
Number of pages12
JournalJournal of Immunological Methods
Volume70
Issue number1
Publication statusPublished - 11 May 1984

Keywords

  • Schistosoma mansoni, Animals, Ovalbumin, Carbodiimides, Humans, Schistosomiasis, Macromolecular Substances, Antigens, Cross-Linking Reagents, Immunoglobulin G, Enzyme-Linked Immunosorbent Assay, Immunoenzyme Techniques, Cricetinae