Abstract
To investigate the effects of antigen cross-linking on ELISA results, we assayed antibodies to several purified antigens, using microtitration trays coated with antigens in their native and cross-linked forms. Cross-linking was achieved by a carbodiimide promoted condensation reaction. For several antigens an increase in sensitivity of the ELISA was obtained after cross-linking. The results of a double antibody sandwich assay using the same cross-linked antigens, showed that the affinity for antibodies remained unchanged after limited cross-linking. Therefore, the higher sensitivity of the ELISA is most likely due to a higher affinity of the cross-linked antigens for the plastic carrier. Cross-linking of proteins in a complex antigen preparation obtained from Schistosoma mansoni led to a large increase in sensitivity.
Original language | English |
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Pages (from-to) | 53-64 |
Number of pages | 12 |
Journal | Journal of Immunological Methods |
Volume | 70 |
Issue number | 1 |
Publication status | Published - 11 May 1984 |
Keywords
- Schistosoma mansoni
- Animals
- Ovalbumin
- Carbodiimides
- Humans
- Schistosomiasis
- Macromolecular Substances
- Antigens
- Cross-Linking Reagents
- Immunoglobulin G
- Enzyme-Linked Immunosorbent Assay
- Immunoenzyme Techniques
- Cricetinae