TY - JOUR
T1 - The death-inducing signalling complex is recruited to lipid rafts in Fas-induced apoptosis
AU - Scheel-Toellner, Dagmar
AU - Wang, K
AU - Singh, R
AU - Majeed, S
AU - Raza, Karim
AU - Curnow, Stephen
AU - Salmon, Michael
AU - Lord, Janet
PY - 2002/10/4
Y1 - 2002/10/4
N2 - Membrane microdomains known as lipid rafts have been shown recently to be involved in Fas signalling and apoptosis in T and B cell lines. Here, we have investigated further the role of lipid rafts in Fas-induced apoptosis in non-transformed human CD4 T cells. We show that Fas-induced apoptosis in CD4 T cells was inhibited by the lipid raft disrupter methyl-beta-cyclodextrin. When lipid rafts were isolated from control and Fas ligand treated cells, we found that a small proportion of Fas was present in the raft fraction in untreated cells and that this was greatly increased upon Fas ligation. The other components of the Death Inducing Signalling Complex (DISC), FADD, and procaspase 8, were also present at higher levels in the raft fraction isolated from Fas ligand treated cells. We conclude that formation of the DISC occurs in lipid rafts and that these membrane microdomains are required for efficient Fas signalling and apoptosis.
AB - Membrane microdomains known as lipid rafts have been shown recently to be involved in Fas signalling and apoptosis in T and B cell lines. Here, we have investigated further the role of lipid rafts in Fas-induced apoptosis in non-transformed human CD4 T cells. We show that Fas-induced apoptosis in CD4 T cells was inhibited by the lipid raft disrupter methyl-beta-cyclodextrin. When lipid rafts were isolated from control and Fas ligand treated cells, we found that a small proportion of Fas was present in the raft fraction in untreated cells and that this was greatly increased upon Fas ligation. The other components of the Death Inducing Signalling Complex (DISC), FADD, and procaspase 8, were also present at higher levels in the raft fraction isolated from Fas ligand treated cells. We conclude that formation of the DISC occurs in lipid rafts and that these membrane microdomains are required for efficient Fas signalling and apoptosis.
KW - DISC
KW - Fas
KW - apoptosis
KW - FADD
KW - lipid raft
UR - http://www.scopus.com/inward/record.url?scp=0036031767&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(02)02311-2
DO - 10.1016/S0006-291X(02)02311-2
M3 - Article
C2 - 12359234
VL - 297
SP - 876
EP - 879
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -