The death-inducing signalling complex is recruited to lipid rafts in Fas-induced apoptosis

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Colleges, School and Institutes

Abstract

Membrane microdomains known as lipid rafts have been shown recently to be involved in Fas signalling and apoptosis in T and B cell lines. Here, we have investigated further the role of lipid rafts in Fas-induced apoptosis in non-transformed human CD4 T cells. We show that Fas-induced apoptosis in CD4 T cells was inhibited by the lipid raft disrupter methyl-beta-cyclodextrin. When lipid rafts were isolated from control and Fas ligand treated cells, we found that a small proportion of Fas was present in the raft fraction in untreated cells and that this was greatly increased upon Fas ligation. The other components of the Death Inducing Signalling Complex (DISC), FADD, and procaspase 8, were also present at higher levels in the raft fraction isolated from Fas ligand treated cells. We conclude that formation of the DISC occurs in lipid rafts and that these membrane microdomains are required for efficient Fas signalling and apoptosis.

Details

Original languageEnglish
Pages (from-to)876-879
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume297
Issue number4
Publication statusPublished - 4 Oct 2002

Keywords

  • DISC, Fas, apoptosis, FADD, lipid raft