The atypical chemokine receptor CCRL1 shapes functional CCL21 gradients in lymph nodes

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The atypical chemokine receptor CCRL1 shapes functional CCL21 gradients in lymph nodes. / Ulvmar, Maria H; Werth, Kathrin; Braun, Asolina; Kelay, Poonam; Hub, Elin; Eller, Kathrin; Chan, Li; Lucas, Beth; Novitzky-Basso, Igor; Nakamura, Kyoko; Rülicke, Thomas; Nibbs, Robert J B; Worbs, Tim; Förster, Reinhold; Rot, Antal.

In: Nature Immunology, Vol. 15, No. 7, 07.2014, p. 623-30.

Research output: Contribution to journalArticlepeer-review

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Ulvmar, MH, Werth, K, Braun, A, Kelay, P, Hub, E, Eller, K, Chan, L, Lucas, B, Novitzky-Basso, I, Nakamura, K, Rülicke, T, Nibbs, RJB, Worbs, T, Förster, R & Rot, A 2014, 'The atypical chemokine receptor CCRL1 shapes functional CCL21 gradients in lymph nodes', Nature Immunology, vol. 15, no. 7, pp. 623-30. https://doi.org/10.1038/ni.2889

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Ulvmar, Maria H ; Werth, Kathrin ; Braun, Asolina ; Kelay, Poonam ; Hub, Elin ; Eller, Kathrin ; Chan, Li ; Lucas, Beth ; Novitzky-Basso, Igor ; Nakamura, Kyoko ; Rülicke, Thomas ; Nibbs, Robert J B ; Worbs, Tim ; Förster, Reinhold ; Rot, Antal. / The atypical chemokine receptor CCRL1 shapes functional CCL21 gradients in lymph nodes. In: Nature Immunology. 2014 ; Vol. 15, No. 7. pp. 623-30.

Bibtex

@article{281d9d107b574ec2bebe6c1c2e82d4b4,
title = "The atypical chemokine receptor CCRL1 shapes functional CCL21 gradients in lymph nodes",
abstract = "Afferent lymph-borne dendritic cells essentially rely on the chemokine receptor CCR7 for their transition from the subcapsular lymph node sinus into the parenchyma, a migratory step driven by putative gradients of CCR7 ligands. We found that lymph node fringes indeed contained physiological gradients of the chemokine CCL21, which depended on the expression of CCRL1, the atypical receptor for the CCR7 ligands CCL19 and CCL21. Lymphatic endothelial cells lining the ceiling of the subcapsular sinus, but not those lining the floor, expressed CCRL1, which scavenged chemokines from the sinus lumen. This created chemokine gradients across the sinus floor and enabled the emigration of dendritic cells. In vitro live imaging revealed that spatially confined expression of CCRL1 was necessary and sufficient for the creation of functional chemokine gradients.",
keywords = "Animals, Cell Movement, Chemokine CCL21, Dendritic Cells, Lymph Nodes, Mice, Mice, Inbred C57BL, Receptors, CCR",
author = "Ulvmar, {Maria H} and Kathrin Werth and Asolina Braun and Poonam Kelay and Elin Hub and Kathrin Eller and Li Chan and Beth Lucas and Igor Novitzky-Basso and Kyoko Nakamura and Thomas R{\"u}licke and Nibbs, {Robert J B} and Tim Worbs and Reinhold F{\"o}rster and Antal Rot",
year = "2014",
month = jul,
doi = "10.1038/ni.2889",
language = "English",
volume = "15",
pages = "623--30",
journal = "Nature Immunology",
issn = "1529-2908",
publisher = "Nature Publishing Group",
number = "7",

}

RIS

TY - JOUR

T1 - The atypical chemokine receptor CCRL1 shapes functional CCL21 gradients in lymph nodes

AU - Ulvmar, Maria H

AU - Werth, Kathrin

AU - Braun, Asolina

AU - Kelay, Poonam

AU - Hub, Elin

AU - Eller, Kathrin

AU - Chan, Li

AU - Lucas, Beth

AU - Novitzky-Basso, Igor

AU - Nakamura, Kyoko

AU - Rülicke, Thomas

AU - Nibbs, Robert J B

AU - Worbs, Tim

AU - Förster, Reinhold

AU - Rot, Antal

PY - 2014/7

Y1 - 2014/7

N2 - Afferent lymph-borne dendritic cells essentially rely on the chemokine receptor CCR7 for their transition from the subcapsular lymph node sinus into the parenchyma, a migratory step driven by putative gradients of CCR7 ligands. We found that lymph node fringes indeed contained physiological gradients of the chemokine CCL21, which depended on the expression of CCRL1, the atypical receptor for the CCR7 ligands CCL19 and CCL21. Lymphatic endothelial cells lining the ceiling of the subcapsular sinus, but not those lining the floor, expressed CCRL1, which scavenged chemokines from the sinus lumen. This created chemokine gradients across the sinus floor and enabled the emigration of dendritic cells. In vitro live imaging revealed that spatially confined expression of CCRL1 was necessary and sufficient for the creation of functional chemokine gradients.

AB - Afferent lymph-borne dendritic cells essentially rely on the chemokine receptor CCR7 for their transition from the subcapsular lymph node sinus into the parenchyma, a migratory step driven by putative gradients of CCR7 ligands. We found that lymph node fringes indeed contained physiological gradients of the chemokine CCL21, which depended on the expression of CCRL1, the atypical receptor for the CCR7 ligands CCL19 and CCL21. Lymphatic endothelial cells lining the ceiling of the subcapsular sinus, but not those lining the floor, expressed CCRL1, which scavenged chemokines from the sinus lumen. This created chemokine gradients across the sinus floor and enabled the emigration of dendritic cells. In vitro live imaging revealed that spatially confined expression of CCRL1 was necessary and sufficient for the creation of functional chemokine gradients.

KW - Animals

KW - Cell Movement

KW - Chemokine CCL21

KW - Dendritic Cells

KW - Lymph Nodes

KW - Mice

KW - Mice, Inbred C57BL

KW - Receptors, CCR

U2 - 10.1038/ni.2889

DO - 10.1038/ni.2889

M3 - Article

C2 - 24813163

VL - 15

SP - 623

EP - 630

JO - Nature Immunology

JF - Nature Immunology

SN - 1529-2908

IS - 7

ER -