The aPKC/Par3/Par6 Polarity Complex and Membrane Order Are Functionally Interdependent in Epithelia During Vertebrate Organogenesis

Research output: Contribution to journalArticlepeer-review

Authors

  • Ahmed Abu-Siniyeh
  • Carola Benzing
  • Silke Rinkwitz
  • Thomas S. Becker
  • Arindam Majumdar
  • Katharina Gaus

Colleges, School and Institutes

Abstract

The differential distribution of lipids between apical and basolateral membranes is necessary for many epithelial cell functions, but how this characteristic membrane organization is integrated within the polarity network during ductal organ development is poorly understood. Here we quantified membrane order in the gut, kidney and liver ductal epithelia in zebrafish larvae at 3-11 days post fertilization (dpf) with Laurdan 2-photon microscopy. We then applied a combination of Laurdan imaging, antisense knock-down and analysis of polarity markers to understand the relationship between membrane order and apical-basal polarity. We found a reciprocal relationship between membrane order and the cell polarity network. Reducing membrane condensation by exogenously added oxysterol or depletion of cholesterol reduced apical targeting of the polarity protein, aPKC. Conversely, using morpholino knock down in zebrafish, we found that membrane order was dependent upon the Crb3 and Par3 polarity protein expression in ductal epithelia. Hence our data suggest that the biophysical property of membrane lipid packing is a regulatory element in apical basal polarity.

Details

Original languageEnglish
Pages (from-to)66-79
Number of pages14
JournalTraffic
Volume17
Issue number1
Early online date23 Nov 2015
Publication statusE-pub ahead of print - 23 Nov 2015

Keywords

  • Apical and basolateral membranes, Epithelial cell, Lipid phases, Membrane organization, Polarity proteins, Zebrafish larvae