The 1st International standard for transforming growth factor-β3 (TGF-β3)

Meenu Wadhwa; Paula Dilger; Michelle Hamill; David Bending; Sarah Gibbs; Guoping Wu; Jo Read; Tony Wyss-Coray; Hui Zhang; John Little; Katherine M Getliffe; Gao Kai; Weihong Wang; David Bender; Chris Bird; Alan B Heath; Anne Cooke; Robin Thorpe

doi:10.1016/j.jim.2012.03.002

The 1st International standard for transforming growth factor-β3 (TGF-β3)

Meenu Wadhwa, Paula Dilger, Michelle Hamill, David Bending, Sarah Gibbs, Guoping Wu, Jo Read, Tony Wyss-Coray, Hui Zhang, John Little, Katherine M Getliffe, Gao Kai, Weihong Wang, David Bender, Chris Bird, Alan B Heath, Anne Cooke, Robin Thorpe

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

One candidate preparation of human sequence recombinant transforming growth factor-β3 (TGF-β3) was formulated and lyophilized at NIBSC prior to evaluation in a collaborative study for its suitability to serve as an international standard. The preparation was tested by 8 laboratories using in vitro bioassays and immunoassays. The candidate preparation 09/234 was judged suitable to serve as an international standard based on the data obtained for biological activity and stability. On the basis of the results reported here, the preparation coded 09/234 was established by the WHO Expert Committee on Biological Standardisation (ECBS) as the WHO 1st IS for human TGF-β3 with an assigned value for TGF-β3 activity of 19,000 IU/ampoule.

Original language	English
Pages (from-to)	1-9
Number of pages	9
Journal	Journal of Immunological Methods
Volume	380
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jim.2012.03.002
Publication status	Published - 29 Jun 2012

Keywords

Biological Assay
Humans
Immunoassay
Reference Standards
Transforming Growth Factor beta3
Journal Article

Access to Document

10.1016/j.jim.2012.03.002

Cite this

Wadhwa, M., Dilger, P., Hamill, M., Bending, D., Gibbs, S., Wu, G., Read, J., Wyss-Coray, T., Zhang, H., Little, J., Getliffe, K. M., Kai, G., Wang, W., Bender, D., Bird, C., Heath, A. B., Cooke, A., & Thorpe, R. (2012). The 1st International standard for transforming growth factor-β3 (TGF-β3). Journal of Immunological Methods, 380(1-2), 1-9. https://doi.org/10.1016/j.jim.2012.03.002

@article{062ebbc70a1f423e93934ad5fa2231ca,

title = "The 1st International standard for transforming growth factor-β3 (TGF-β3)",

abstract = "One candidate preparation of human sequence recombinant transforming growth factor-β3 (TGF-β3) was formulated and lyophilized at NIBSC prior to evaluation in a collaborative study for its suitability to serve as an international standard. The preparation was tested by 8 laboratories using in vitro bioassays and immunoassays. The candidate preparation 09/234 was judged suitable to serve as an international standard based on the data obtained for biological activity and stability. On the basis of the results reported here, the preparation coded 09/234 was established by the WHO Expert Committee on Biological Standardisation (ECBS) as the WHO 1st IS for human TGF-β3 with an assigned value for TGF-β3 activity of 19,000 IU/ampoule.",

keywords = "Biological Assay, Humans, Immunoassay, Reference Standards, Transforming Growth Factor beta3, Journal Article",

author = "Meenu Wadhwa and Paula Dilger and Michelle Hamill and David Bending and Sarah Gibbs and Guoping Wu and Jo Read and Tony Wyss-Coray and Hui Zhang and John Little and Getliffe, {Katherine M} and Gao Kai and Weihong Wang and David Bender and Chris Bird and Heath, {Alan B} and Anne Cooke and Robin Thorpe",

year = "2012",

month = jun,

day = "29",

doi = "10.1016/j.jim.2012.03.002",

language = "English",

volume = "380",

pages = "1--9",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier",

number = "1-2",

}

Wadhwa, M, Dilger, P, Hamill, M, Bending, D, Gibbs, S, Wu, G, Read, J, Wyss-Coray, T, Zhang, H, Little, J, Getliffe, KM, Kai, G, Wang, W, Bender, D, Bird, C, Heath, AB, Cooke, A & Thorpe, R 2012, 'The 1st International standard for transforming growth factor-β3 (TGF-β3)', Journal of Immunological Methods, vol. 380, no. 1-2, pp. 1-9. https://doi.org/10.1016/j.jim.2012.03.002

TY - JOUR

T1 - The 1st International standard for transforming growth factor-β3 (TGF-β3)

AU - Wadhwa, Meenu

AU - Dilger, Paula

AU - Hamill, Michelle

AU - Bending, David

AU - Gibbs, Sarah

AU - Wu, Guoping

AU - Read, Jo

AU - Wyss-Coray, Tony

AU - Zhang, Hui

AU - Little, John

AU - Getliffe, Katherine M

AU - Kai, Gao

AU - Wang, Weihong

AU - Bender, David

AU - Bird, Chris

AU - Heath, Alan B

AU - Cooke, Anne

AU - Thorpe, Robin

PY - 2012/6/29

Y1 - 2012/6/29

N2 - One candidate preparation of human sequence recombinant transforming growth factor-β3 (TGF-β3) was formulated and lyophilized at NIBSC prior to evaluation in a collaborative study for its suitability to serve as an international standard. The preparation was tested by 8 laboratories using in vitro bioassays and immunoassays. The candidate preparation 09/234 was judged suitable to serve as an international standard based on the data obtained for biological activity and stability. On the basis of the results reported here, the preparation coded 09/234 was established by the WHO Expert Committee on Biological Standardisation (ECBS) as the WHO 1st IS for human TGF-β3 with an assigned value for TGF-β3 activity of 19,000 IU/ampoule.

AB - One candidate preparation of human sequence recombinant transforming growth factor-β3 (TGF-β3) was formulated and lyophilized at NIBSC prior to evaluation in a collaborative study for its suitability to serve as an international standard. The preparation was tested by 8 laboratories using in vitro bioassays and immunoassays. The candidate preparation 09/234 was judged suitable to serve as an international standard based on the data obtained for biological activity and stability. On the basis of the results reported here, the preparation coded 09/234 was established by the WHO Expert Committee on Biological Standardisation (ECBS) as the WHO 1st IS for human TGF-β3 with an assigned value for TGF-β3 activity of 19,000 IU/ampoule.

KW - Biological Assay

KW - Humans

KW - Immunoassay

KW - Reference Standards

KW - Transforming Growth Factor beta3

KW - Journal Article

U2 - 10.1016/j.jim.2012.03.002

DO - 10.1016/j.jim.2012.03.002

M3 - Article

C2 - 22464938

SN - 0022-1759

VL - 380

SP - 1

EP - 9

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1-2

ER -

The 1st International standard for transforming growth factor-β3 (TGF-β3)

Abstract

Keywords

Access to Document

Fingerprint

Cite this