Termination of Eukaryotic Replication Forks

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Authors

Colleges, School and Institutes

External organisations

  • Institute of Cancer and Genomic Sciences, University of Birmingham, Birmingham, UK. a.gambus@bham.ac.uk.

Abstract

Termination of DNA replication forks takes place when two replication forks coming from neighbouring origins meet each other usually in the midpoint of the replicon. At this stage, the remaining fragments of DNA have to be unwound, all remaining DNA replicated and newly synthesised strands ligated to produce continuous sister chromatids. Finally, the replication machinery has to be taken off, chromatin re-assembled, and entwisted sister chromatids resolved topologically.Over the last few decades, we have learned a lot about the assembly of the helicase and replisome and the initiation stage of DNA replication. We also know much more about the ability of forks to cope with replication stress. However, only within recent years we have gained the first glimpse of the mechanism of replication fork termination. In this chapter I will summarise the recent findings on replication termination, weigh this against the past literature and discuss relevant consequences and views for the future.

Details

Original languageEnglish
Title of host publicationDNA Replication
Subtitle of host publicationFrom Old Principles to New Discoveries
Publication statusPublished - 31 Jan 2018

Publication series

NameAdvances in Experimental Medicine and Biology
PublisherSpringer
Volume1042
ISSN (Print)0065-2598