Targeting choroid plexus epithelia and ventricular ependyma for drug delivery to the central nervous system.

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Targeting choroid plexus epithelia and ventricular ependyma for drug delivery to the central nervous system. / Gonzalez, Ana; Leadbeater, Wendy; Burg, M; Sims, K; Terasaki, T; Johanson, CE; Stopa, EG; Eliceiri, BP; Baird, Andrew.

In: BMC Neuroscience, Vol. 12, 4, 07.01.2011.

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@article{d00352b866af4128805ed03630768e3b,
title = "Targeting choroid plexus epithelia and ventricular ependyma for drug delivery to the central nervous system.",
abstract = "BACKGROUND: Because the choroid plexus (CP) is uniquely suited to control the composition of cerebrospinal fluid (CSF), there may be therapeutic benefits to increasing the levels of biologically active proteins in CSF to modulate central nervous system (CNS) functions. To this end, we sought to identify peptides capable of ligand-mediated targeting to CP epithelial cells reasoning that they could be exploited to deliver drugs, biotherapeutics and genes to the CNS. METHODS: A peptide library displayed on M13 bacteriophage was screened for ligands capable of internalizing into CP epithelial cells by incubating phage with CP explants for 2 hours at 37C and recovering particles with targeting capacity.RESULTS: Three peptides, identified after four rounds of screening, were analyzed for specific and dose dependent binding and internalization. Binding was deemed specific because internalization was prevented by co-incubation with cognate synthetic peptides. Furthermore, after i.c.v. injection into rat brains, each peptide was found to target phage to epithelial cells in CP and to ependyma lining the ventricles.CONCLUSION: These data demonstrate that ligand-mediated targeting can be used as a strategy for drug delivery to the central nervous system and opens the possibility of using the choroid plexus as a portal of entry into the brain.",
author = "Ana Gonzalez and Wendy Leadbeater and M Burg and K Sims and T Terasaki and CE Johanson and EG Stopa and BP Eliceiri and Andrew Baird",
year = "2011",
month = jan
day = "7",
doi = "10.1186/1471-2202-12-4",
language = "English",
volume = "12",
journal = "BMC Neuroscience",
issn = "1471-2202",
publisher = "Springer",

}

RIS

TY - JOUR

T1 - Targeting choroid plexus epithelia and ventricular ependyma for drug delivery to the central nervous system.

AU - Gonzalez, Ana

AU - Leadbeater, Wendy

AU - Burg, M

AU - Sims, K

AU - Terasaki, T

AU - Johanson, CE

AU - Stopa, EG

AU - Eliceiri, BP

AU - Baird, Andrew

PY - 2011/1/7

Y1 - 2011/1/7

N2 - BACKGROUND: Because the choroid plexus (CP) is uniquely suited to control the composition of cerebrospinal fluid (CSF), there may be therapeutic benefits to increasing the levels of biologically active proteins in CSF to modulate central nervous system (CNS) functions. To this end, we sought to identify peptides capable of ligand-mediated targeting to CP epithelial cells reasoning that they could be exploited to deliver drugs, biotherapeutics and genes to the CNS. METHODS: A peptide library displayed on M13 bacteriophage was screened for ligands capable of internalizing into CP epithelial cells by incubating phage with CP explants for 2 hours at 37C and recovering particles with targeting capacity.RESULTS: Three peptides, identified after four rounds of screening, were analyzed for specific and dose dependent binding and internalization. Binding was deemed specific because internalization was prevented by co-incubation with cognate synthetic peptides. Furthermore, after i.c.v. injection into rat brains, each peptide was found to target phage to epithelial cells in CP and to ependyma lining the ventricles.CONCLUSION: These data demonstrate that ligand-mediated targeting can be used as a strategy for drug delivery to the central nervous system and opens the possibility of using the choroid plexus as a portal of entry into the brain.

AB - BACKGROUND: Because the choroid plexus (CP) is uniquely suited to control the composition of cerebrospinal fluid (CSF), there may be therapeutic benefits to increasing the levels of biologically active proteins in CSF to modulate central nervous system (CNS) functions. To this end, we sought to identify peptides capable of ligand-mediated targeting to CP epithelial cells reasoning that they could be exploited to deliver drugs, biotherapeutics and genes to the CNS. METHODS: A peptide library displayed on M13 bacteriophage was screened for ligands capable of internalizing into CP epithelial cells by incubating phage with CP explants for 2 hours at 37C and recovering particles with targeting capacity.RESULTS: Three peptides, identified after four rounds of screening, were analyzed for specific and dose dependent binding and internalization. Binding was deemed specific because internalization was prevented by co-incubation with cognate synthetic peptides. Furthermore, after i.c.v. injection into rat brains, each peptide was found to target phage to epithelial cells in CP and to ependyma lining the ventricles.CONCLUSION: These data demonstrate that ligand-mediated targeting can be used as a strategy for drug delivery to the central nervous system and opens the possibility of using the choroid plexus as a portal of entry into the brain.

U2 - 10.1186/1471-2202-12-4

DO - 10.1186/1471-2202-12-4

M3 - Article

C2 - 21214926

VL - 12

JO - BMC Neuroscience

JF - BMC Neuroscience

SN - 1471-2202

M1 - 4

ER -