tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging

Research output: Contribution to journalArticle

Authors

  • Geva Hilzenrat
  • Jason Tran
  • Zhengmin Yang
  • Hugh H MacRae
  • Matthew A B Baker
  • J Justin Gooding
  • Katharina Gaus

Colleges, School and Institutes

External organisations

  • EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
  • ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales, Sydney, NSW 2052, Australia.
  • Commonwealth Scientific and Industrial Research Organisation (CSIRO), Synthetic Biology Future Science Platform, Brisbane, Australia.
  • Australian Centre for NanoMedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of New South Wales, Sydney, NSW 2052, Australia.

Abstract

Recently, DNA-PAINT single-molecule localization microscopy (SMLM) has shown great promise for quantitative imaging; however, labelling strategies thus far have relied on multivalent and affinity-based approaches. Here, the covalent labelling of expressed protein tags (SNAP tag and Halo tag) with single DNA-docking strands and application of SMLM via DNA-PAINT is demonstrated. tagPAINT is then used for T-cell receptor signalling proteins at the immune synapse as a proof of principle.

Details

Original languageEnglish
Article number191268
JournalRoyal Society Open Science
Volume6
Issue number12
Publication statusPublished - 11 Dec 2019

Keywords

  • protein tags, monovalent, covalent, DNA-PAINT imaging, superresolution