tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging
Research output: Contribution to journal › Article
Colleges, School and Institutes
- EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
- ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales, Sydney, NSW 2052, Australia.
- Commonwealth Scientific and Industrial Research Organisation (CSIRO), Synthetic Biology Future Science Platform, Brisbane, Australia.
- Australian Centre for NanoMedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of New South Wales, Sydney, NSW 2052, Australia.
Recently, DNA-PAINT single-molecule localization microscopy (SMLM) has shown great promise for quantitative imaging; however, labelling strategies thus far have relied on multivalent and affinity-based approaches. Here, the covalent labelling of expressed protein tags (SNAP tag and Halo tag) with single DNA-docking strands and application of SMLM via DNA-PAINT is demonstrated. tagPAINT is then used for T-cell receptor signalling proteins at the immune synapse as a proof of principle.
|Journal||Royal Society Open Science|
|Publication status||Published - 11 Dec 2019|
- protein tags, monovalent, covalent, DNA-PAINT imaging, superresolution