Research output per year
Research output per year
Daniel J Nieves, Geva Hilzenrat, Jason Tran, Zhengmin Yang, Hugh H MacRae, Matthew A B Baker, J Justin Gooding, Katharina Gaus
Research output: Contribution to journal › Article › peer-review
Recently, DNA-PAINT single-molecule localization microscopy (SMLM) has shown great promise for quantitative imaging; however, labelling strategies thus far have relied on multivalent and affinity-based approaches. Here, the covalent labelling of expressed protein tags (SNAP tag and Halo tag) with single DNA-docking strands and application of SMLM via DNA-PAINT is demonstrated. tagPAINT is then used for T-cell receptor signalling proteins at the immune synapse as a proof of principle.
Original language | English |
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Article number | 191268 |
Journal | Royal Society Open Science |
Volume | 6 |
Issue number | 12 |
DOIs | |
Publication status | Published - 11 Dec 2019 |
Research output: Contribution to journal › Article › peer-review