T cell memory response to pneumococcal protein antigens in an area of high pneumococcal carriage and disease

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T cell memory response to pneumococcal protein antigens in an area of high pneumococcal carriage and disease. / Mureithi, Marianne W; Finn, Adam; Ota, Martin O; Zhang, Qibo; Davenport, Victoria; Mitchell, Timothy J; Williams, Neil A; Adegbola, Richard A; Heyderman, Robert S.

In: The Journal of Infectious Diseases, Vol. 200, No. 5, 01.09.2009, p. 783-93.

Research output: Contribution to journalArticlepeer-review

Harvard

Mureithi, MW, Finn, A, Ota, MO, Zhang, Q, Davenport, V, Mitchell, TJ, Williams, NA, Adegbola, RA & Heyderman, RS 2009, 'T cell memory response to pneumococcal protein antigens in an area of high pneumococcal carriage and disease', The Journal of Infectious Diseases, vol. 200, no. 5, pp. 783-93. https://doi.org/10.1086/605023

APA

Mureithi, M. W., Finn, A., Ota, M. O., Zhang, Q., Davenport, V., Mitchell, T. J., Williams, N. A., Adegbola, R. A., & Heyderman, R. S. (2009). T cell memory response to pneumococcal protein antigens in an area of high pneumococcal carriage and disease. The Journal of Infectious Diseases, 200(5), 783-93. https://doi.org/10.1086/605023

Vancouver

Author

Mureithi, Marianne W ; Finn, Adam ; Ota, Martin O ; Zhang, Qibo ; Davenport, Victoria ; Mitchell, Timothy J ; Williams, Neil A ; Adegbola, Richard A ; Heyderman, Robert S. / T cell memory response to pneumococcal protein antigens in an area of high pneumococcal carriage and disease. In: The Journal of Infectious Diseases. 2009 ; Vol. 200, No. 5. pp. 783-93.

Bibtex

@article{b94cbb5c72e141a0817f9baa702ad8cb,
title = "T cell memory response to pneumococcal protein antigens in an area of high pneumococcal carriage and disease",
abstract = "BACKGROUND: Streptococcus pneumoniae is a leading cause of vaccine-preventable disease worldwide. Pneumococcal protein antigens are currently under study as components of potential vaccines that offer protection against multiple serotypes. We have therefore characterized T cell pneumococcal immunity acquired through asymptomatic carriage.METHODS: Peripheral blood mononuclear cells from 40 healthy Gambian adults were stimulated with supernatants derived from S. pneumoniae strain (D39), 2 isogenic mutant strains lacking either pneumolysin or choline binding protein A, and recombinant pneumolysin. Immune responses were measured by cellular proliferation and by interleukin-10 (IL-10) and interferon-gamma (IFN-gamma) enzyme-linked immunosorbent spot and bioplex cytokine assays. Nasopharyngeal swabs were cultured to determine carriage rates.RESULTS: S. pneumoniae nasopharyngeal carriage was detected in 60% of individuals. Both effector and resting (or central) CD4(+) T cell memory were frequently present to a range of pneumococcal antigens. However, the level of the effector memory response did not relate to current nasopharyngeal carriage. Pneumolysin was not immunodominant in these T cell responses but induced a distinct proinflammatory profile (high IFN-gamma, IL-12[p40], and L-17 levels and low IL-10 and IL-13 levels).CONCLUSIONS: In this population, T cell-mediated immunological memory potentially capable of pathogen clearance and immune surveillance is common but is not associated with the absolute interruption of pneumococcal carriage. How this naturally acquired immune memory influences pneumococcal vaccine efficacy remains to be determined.",
keywords = "Adolescent, Adult, Animals, Antigens, Bacterial, Bacterial Proteins, Blood, Carrier State, Cell Proliferation, Cells, Cultured, Gambia, Humans, Immunologic Memory, Interferon-gamma, Interleukin-10, Male, Middle Aged, Nasopharynx, Pneumococcal Infections, Streptococcus pneumoniae, T-Lymphocytes, Young Adult",
author = "Mureithi, {Marianne W} and Adam Finn and Ota, {Martin O} and Qibo Zhang and Victoria Davenport and Mitchell, {Timothy J} and Williams, {Neil A} and Adegbola, {Richard A} and Heyderman, {Robert S}",
year = "2009",
month = sep,
day = "1",
doi = "10.1086/605023",
language = "English",
volume = "200",
pages = "783--93",
journal = "The Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "5",

}

RIS

TY - JOUR

T1 - T cell memory response to pneumococcal protein antigens in an area of high pneumococcal carriage and disease

AU - Mureithi, Marianne W

AU - Finn, Adam

AU - Ota, Martin O

AU - Zhang, Qibo

AU - Davenport, Victoria

AU - Mitchell, Timothy J

AU - Williams, Neil A

AU - Adegbola, Richard A

AU - Heyderman, Robert S

PY - 2009/9/1

Y1 - 2009/9/1

N2 - BACKGROUND: Streptococcus pneumoniae is a leading cause of vaccine-preventable disease worldwide. Pneumococcal protein antigens are currently under study as components of potential vaccines that offer protection against multiple serotypes. We have therefore characterized T cell pneumococcal immunity acquired through asymptomatic carriage.METHODS: Peripheral blood mononuclear cells from 40 healthy Gambian adults were stimulated with supernatants derived from S. pneumoniae strain (D39), 2 isogenic mutant strains lacking either pneumolysin or choline binding protein A, and recombinant pneumolysin. Immune responses were measured by cellular proliferation and by interleukin-10 (IL-10) and interferon-gamma (IFN-gamma) enzyme-linked immunosorbent spot and bioplex cytokine assays. Nasopharyngeal swabs were cultured to determine carriage rates.RESULTS: S. pneumoniae nasopharyngeal carriage was detected in 60% of individuals. Both effector and resting (or central) CD4(+) T cell memory were frequently present to a range of pneumococcal antigens. However, the level of the effector memory response did not relate to current nasopharyngeal carriage. Pneumolysin was not immunodominant in these T cell responses but induced a distinct proinflammatory profile (high IFN-gamma, IL-12[p40], and L-17 levels and low IL-10 and IL-13 levels).CONCLUSIONS: In this population, T cell-mediated immunological memory potentially capable of pathogen clearance and immune surveillance is common but is not associated with the absolute interruption of pneumococcal carriage. How this naturally acquired immune memory influences pneumococcal vaccine efficacy remains to be determined.

AB - BACKGROUND: Streptococcus pneumoniae is a leading cause of vaccine-preventable disease worldwide. Pneumococcal protein antigens are currently under study as components of potential vaccines that offer protection against multiple serotypes. We have therefore characterized T cell pneumococcal immunity acquired through asymptomatic carriage.METHODS: Peripheral blood mononuclear cells from 40 healthy Gambian adults were stimulated with supernatants derived from S. pneumoniae strain (D39), 2 isogenic mutant strains lacking either pneumolysin or choline binding protein A, and recombinant pneumolysin. Immune responses were measured by cellular proliferation and by interleukin-10 (IL-10) and interferon-gamma (IFN-gamma) enzyme-linked immunosorbent spot and bioplex cytokine assays. Nasopharyngeal swabs were cultured to determine carriage rates.RESULTS: S. pneumoniae nasopharyngeal carriage was detected in 60% of individuals. Both effector and resting (or central) CD4(+) T cell memory were frequently present to a range of pneumococcal antigens. However, the level of the effector memory response did not relate to current nasopharyngeal carriage. Pneumolysin was not immunodominant in these T cell responses but induced a distinct proinflammatory profile (high IFN-gamma, IL-12[p40], and L-17 levels and low IL-10 and IL-13 levels).CONCLUSIONS: In this population, T cell-mediated immunological memory potentially capable of pathogen clearance and immune surveillance is common but is not associated with the absolute interruption of pneumococcal carriage. How this naturally acquired immune memory influences pneumococcal vaccine efficacy remains to be determined.

KW - Adolescent

KW - Adult

KW - Animals

KW - Antigens, Bacterial

KW - Bacterial Proteins

KW - Blood

KW - Carrier State

KW - Cell Proliferation

KW - Cells, Cultured

KW - Gambia

KW - Humans

KW - Immunologic Memory

KW - Interferon-gamma

KW - Interleukin-10

KW - Male

KW - Middle Aged

KW - Nasopharynx

KW - Pneumococcal Infections

KW - Streptococcus pneumoniae

KW - T-Lymphocytes

KW - Young Adult

U2 - 10.1086/605023

DO - 10.1086/605023

M3 - Article

C2 - 19642930

VL - 200

SP - 783

EP - 793

JO - The Journal of Infectious Diseases

JF - The Journal of Infectious Diseases

SN - 0022-1899

IS - 5

ER -