Synthesis of a Novel alpha-Galactosyl Ceramide Haptenated-Lipid Antigen, a Useful Tool in Demonstrating the Involvement of iNKT Cells in the Production of Antilipid Antibodies

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@article{f226b67eb543480cbf8ab976b12843b2,
title = "Synthesis of a Novel alpha-Galactosyl Ceramide Haptenated-Lipid Antigen, a Useful Tool in Demonstrating the Involvement of iNKT Cells in the Production of Antilipid Antibodies",
abstract = "A new haptenated derivative of a-galactosyl ceramide (alpha-GalCer) has been synthesized to assist in the study of the mechanism of T cell help for the production of B cell antibodies. Our synthetic route provides access to an amine intermediate which can easily be extended to generate an array of compounds, useful in various ongoing studies. Herein, we also describe the biological evaluation of the nitrophenyl (NP) haptenated alpha-GalCer and demonstrate its importance in such mechanistic studies. For instance, ill vitro studies showed that NP-alpha-GalCer stimulates both T and B cell proliferation while in vivo studies in immunized mice showed the production of IgG anti-NP antibodies after exposure to NP-alpha-GalCer. The interpretation of these results helps toward a better understanding of T cell help for the production of antibodies.",
author = "Natacha Veerapen and EA Leadbetter and MB Brenner and Liam Cox and Gurdyal Besra",
year = "2010",
month = apr,
day = "1",
doi = "10.1021/bc9005255",
language = "English",
volume = "21",
pages = "741--747",
journal = "Bioconjugate Chemistry",
issn = "1043-1802",
publisher = "American Chemical Society",
number = "4",

}

RIS

TY - JOUR

T1 - Synthesis of a Novel alpha-Galactosyl Ceramide Haptenated-Lipid Antigen, a Useful Tool in Demonstrating the Involvement of iNKT Cells in the Production of Antilipid Antibodies

AU - Veerapen, Natacha

AU - Leadbetter, EA

AU - Brenner, MB

AU - Cox, Liam

AU - Besra, Gurdyal

PY - 2010/4/1

Y1 - 2010/4/1

N2 - A new haptenated derivative of a-galactosyl ceramide (alpha-GalCer) has been synthesized to assist in the study of the mechanism of T cell help for the production of B cell antibodies. Our synthetic route provides access to an amine intermediate which can easily be extended to generate an array of compounds, useful in various ongoing studies. Herein, we also describe the biological evaluation of the nitrophenyl (NP) haptenated alpha-GalCer and demonstrate its importance in such mechanistic studies. For instance, ill vitro studies showed that NP-alpha-GalCer stimulates both T and B cell proliferation while in vivo studies in immunized mice showed the production of IgG anti-NP antibodies after exposure to NP-alpha-GalCer. The interpretation of these results helps toward a better understanding of T cell help for the production of antibodies.

AB - A new haptenated derivative of a-galactosyl ceramide (alpha-GalCer) has been synthesized to assist in the study of the mechanism of T cell help for the production of B cell antibodies. Our synthetic route provides access to an amine intermediate which can easily be extended to generate an array of compounds, useful in various ongoing studies. Herein, we also describe the biological evaluation of the nitrophenyl (NP) haptenated alpha-GalCer and demonstrate its importance in such mechanistic studies. For instance, ill vitro studies showed that NP-alpha-GalCer stimulates both T and B cell proliferation while in vivo studies in immunized mice showed the production of IgG anti-NP antibodies after exposure to NP-alpha-GalCer. The interpretation of these results helps toward a better understanding of T cell help for the production of antibodies.

U2 - 10.1021/bc9005255

DO - 10.1021/bc9005255

M3 - Article

C2 - 20345125

VL - 21

SP - 741

EP - 747

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

SN - 1043-1802

IS - 4

ER -