Synthesis of a Novel alpha-Galactosyl Ceramide Haptenated-Lipid Antigen, a Useful Tool in Demonstrating the Involvement of iNKT Cells in the Production of Antilipid Antibodies

Research output: Contribution to journalArticle

Authors

Colleges, School and Institutes

Abstract

A new haptenated derivative of a-galactosyl ceramide (alpha-GalCer) has been synthesized to assist in the study of the mechanism of T cell help for the production of B cell antibodies. Our synthetic route provides access to an amine intermediate which can easily be extended to generate an array of compounds, useful in various ongoing studies. Herein, we also describe the biological evaluation of the nitrophenyl (NP) haptenated alpha-GalCer and demonstrate its importance in such mechanistic studies. For instance, ill vitro studies showed that NP-alpha-GalCer stimulates both T and B cell proliferation while in vivo studies in immunized mice showed the production of IgG anti-NP antibodies after exposure to NP-alpha-GalCer. The interpretation of these results helps toward a better understanding of T cell help for the production of antibodies.

Details

Original languageEnglish
Pages (from-to)741-747
Number of pages7
JournalBioconjugate Chemistry
Volume21
Issue number4
Publication statusPublished - 1 Apr 2010