Synergic interplay of the La motif, RRM1 and the interdomain linker of LARP6 in the recognition of collagen mRNA expands the RNA binding repertoire of the La module

Research output: Contribution to journalArticlepeer-review


  • Luigi Martino
  • Simon Pennell
  • Geoff Kelly
  • Baptiste Busi
  • Paul Brown
  • R Andrew Atkinson
  • Nicholas J H Salisbury
  • Zi-Hao Ooi
  • Kang-Wei See
  • Caterina Alfano
  • Tam T T Bui
  • Maria R Conte

Colleges, School and Institutes

External organisations

  • King’s College London
  • Division of Molecular Structure, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK.
  • MRC Biomedical NMR Centre, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK.
  • École Normale Supérieure de Lyon
  • Randall Division of Cell and Molecular Biophysics, King's College London, New Hunt's House, Guy's Campus, London SE1 1UL, UK Department of Biological Sciences, National University of Singapore, Singapore 117543.


The La-related proteins (LARPs) form a diverse group of RNA-binding proteins characterized by the possession of a composite RNA binding unit, the La module. The La module comprises two domains, the La motif (LaM) and the RRM1, which together recognize and bind to a wide array of RNA substrates. Structural information regarding the La module is at present restricted to the prototypic La protein, which acts as an RNA chaperone binding to 3' UUUOH sequences of nascent RNA polymerase III transcripts. In contrast, LARP6 is implicated in the regulation of collagen synthesis and interacts with a specific stem-loop within the 5' UTR of the collagen mRNA. Here, we present the structure of the LaM and RRM1 of human LARP6 uncovering in both cases considerable structural variation in comparison to the equivalent domains in La and revealing an unprecedented fold for the RRM1. A mutagenic study guided by the structures revealed that RNA recognition requires synergy between the LaM and RRM1 as well as the participation of the interdomain linker, probably in realizing tandem domain configurations and dynamics required for substrate selectivity. Our study highlights a considerable complexity and plasticity in the architecture of the La module within LARPs.

Bibliographic note

© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.


Original languageEnglish
Pages (from-to)645-660
Number of pages16
JournalNucleic Acids Research
Issue number1
Early online date8 Dec 2014
Publication statusPublished - 9 Jan 2015


  • 5' Untranslated Regions, Amino Acid Motifs, Amino Acid Sequence, Autoantigens/chemistry, Collagen/genetics, Humans, Models, Molecular, Molecular Sequence Data, Mutation, Protein Binding, Ribonucleoproteins/chemistry, Sequence Alignment