Surfaceome interrogation using an RNA-seq approach highlights leukemia initiating cell biomarkers in an LMO2 T cell transgenic model
Research output: Contribution to journal › Article › peer-review
Authors
Colleges, School and Institutes
External organisations
- Trivago GmbH
- 199
- Weatherall Institute of Molecular Medicine
- Wellcome Trust Centre for Human Genetics
- Institute of Cancer and Genomic Sciences
- University Hospital A.O.
Abstract
The surfaceome is critical because surface proteins provide a gateway for internal signals and transfer of molecules into cells, and surfaceome differences can influence therapy response. We have used a surfaceome analysis method, based on comparing RNA-seq data between normal and abnormal cells (Surfaceome DataBase Mining or Surfaceome DBM), to identify sets of upregulated cell surface protein mRNAs in an LMO2-mediated T-ALL mouse model and corroborated by protein detection using antibodies. In this model the leukemia initiating cells (LICs) comprise pre-leukaemic, differentiation inhibited thymocytes allowing us to provide a profile of the LIC surfaceome in which GPR56, CD53 and CD59a are co-expressed with CD25. Implementation of cell surface interaction assays demonstrates fluid interaction of surface proteins and CD25 is only internalized when co-localized with other proteins. The Surfaceome DBM approach to analyse cancer cell surfaceomes is a way to find targetable surface biomarkers for clinical conditions where RNA-seq data from normal and abnormal cell are available.
Details
Original language | English |
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Article number | 5760 |
Journal | Scientific Reports |
Volume | 9 |
Issue number | 1 |
Publication status | Published - 8 Apr 2019 |