Surface chemistry of photoluminescent F8BT conjugated polymer nanoparticles determines protein corona formation and internalization by phagocytic cells

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Surface chemistry of photoluminescent F8BT conjugated polymer nanoparticles determines protein corona formation and internalization by phagocytic cells. / Ahmad Khanbeigi, Raha; Abelha, Thais Fedatto; Woods, Arcadia; Rastoin, Olivia; Harvey, Richard D; Jones, Marie-Christine; Forbes, Ben; Green, Mark A; Collins, Helen; Dailey, Lea Ann.

In: Biomacromolecules, Vol. 16, No. 3, 09.03.2015, p. 733-42.

Research output: Contribution to journalArticlepeer-review

Harvard

Ahmad Khanbeigi, R, Abelha, TF, Woods, A, Rastoin, O, Harvey, RD, Jones, M-C, Forbes, B, Green, MA, Collins, H & Dailey, LA 2015, 'Surface chemistry of photoluminescent F8BT conjugated polymer nanoparticles determines protein corona formation and internalization by phagocytic cells', Biomacromolecules, vol. 16, no. 3, pp. 733-42. https://doi.org/10.1021/bm501649y

APA

Ahmad Khanbeigi, R., Abelha, T. F., Woods, A., Rastoin, O., Harvey, R. D., Jones, M-C., Forbes, B., Green, M. A., Collins, H., & Dailey, L. A. (2015). Surface chemistry of photoluminescent F8BT conjugated polymer nanoparticles determines protein corona formation and internalization by phagocytic cells. Biomacromolecules, 16(3), 733-42. https://doi.org/10.1021/bm501649y

Vancouver

Author

Ahmad Khanbeigi, Raha ; Abelha, Thais Fedatto ; Woods, Arcadia ; Rastoin, Olivia ; Harvey, Richard D ; Jones, Marie-Christine ; Forbes, Ben ; Green, Mark A ; Collins, Helen ; Dailey, Lea Ann. / Surface chemistry of photoluminescent F8BT conjugated polymer nanoparticles determines protein corona formation and internalization by phagocytic cells. In: Biomacromolecules. 2015 ; Vol. 16, No. 3. pp. 733-42.

Bibtex

@article{59c3410525ce45eeac585d1fc58b0a6b,
title = "Surface chemistry of photoluminescent F8BT conjugated polymer nanoparticles determines protein corona formation and internalization by phagocytic cells",
abstract = "Conjugated polymer nanoparticles are being developed for a variety of diagnostic and theranostic applications. The conjugated polymer, F8BT, a polyfluorene derivative, was used as a model system to examine the biological behavior of conjugated polymer nanoparticle formulations stabilized with ionic (sodium dodecyl sulfate; F8BT-SDS; ∼207 nm; -31 mV) and nonionic (pegylated 12-hydroxystearate; F8BT-PEG; ∼175 nm; -5 mV) surfactants, and compared with polystyrene nanoparticles of a similar size (PS200; ∼217 nm; -40 mV). F8BT nanoparticles were as hydrophobic as PS200 (hydrophobic interaction chromatography index value: 0.96) and showed evidence of protein corona formation after incubation with serum-containing medium; however, unlike polystyrene, F8BT nanoparticles did not enrich specific proteins onto the nanoparticle surface. J774A.1 macrophage cells internalized approximately ∼20% and ∼60% of the F8BT-SDS and PS200 delivered dose (calculated by the ISDD model) in serum-supplemented and serum-free conditions, respectively, while cell association of F8BT-PEG was minimal (<5% of the delivered dose). F8BT-PEG, however, was more cytotoxic (IC50 4.5 μg cm(-2)) than F8BT-SDS or PS200. The study results highlight that F8BT surface chemistry influences the composition of the protein corona, while the properties of the conjugated polymer nanoparticle surfactant stabilizer used determine particle internalization and biocompatibility profile.",
author = "{Ahmad Khanbeigi}, Raha and Abelha, {Thais Fedatto} and Arcadia Woods and Olivia Rastoin and Harvey, {Richard D} and Marie-Christine Jones and Ben Forbes and Green, {Mark A} and Helen Collins and Dailey, {Lea Ann}",
year = "2015",
month = mar,
day = "9",
doi = "10.1021/bm501649y",
language = "English",
volume = "16",
pages = "733--42",
journal = "Biomacromolecules",
issn = "1525-7797",
publisher = "American Chemical Society",
number = "3",

}

RIS

TY - JOUR

T1 - Surface chemistry of photoluminescent F8BT conjugated polymer nanoparticles determines protein corona formation and internalization by phagocytic cells

AU - Ahmad Khanbeigi, Raha

AU - Abelha, Thais Fedatto

AU - Woods, Arcadia

AU - Rastoin, Olivia

AU - Harvey, Richard D

AU - Jones, Marie-Christine

AU - Forbes, Ben

AU - Green, Mark A

AU - Collins, Helen

AU - Dailey, Lea Ann

PY - 2015/3/9

Y1 - 2015/3/9

N2 - Conjugated polymer nanoparticles are being developed for a variety of diagnostic and theranostic applications. The conjugated polymer, F8BT, a polyfluorene derivative, was used as a model system to examine the biological behavior of conjugated polymer nanoparticle formulations stabilized with ionic (sodium dodecyl sulfate; F8BT-SDS; ∼207 nm; -31 mV) and nonionic (pegylated 12-hydroxystearate; F8BT-PEG; ∼175 nm; -5 mV) surfactants, and compared with polystyrene nanoparticles of a similar size (PS200; ∼217 nm; -40 mV). F8BT nanoparticles were as hydrophobic as PS200 (hydrophobic interaction chromatography index value: 0.96) and showed evidence of protein corona formation after incubation with serum-containing medium; however, unlike polystyrene, F8BT nanoparticles did not enrich specific proteins onto the nanoparticle surface. J774A.1 macrophage cells internalized approximately ∼20% and ∼60% of the F8BT-SDS and PS200 delivered dose (calculated by the ISDD model) in serum-supplemented and serum-free conditions, respectively, while cell association of F8BT-PEG was minimal (<5% of the delivered dose). F8BT-PEG, however, was more cytotoxic (IC50 4.5 μg cm(-2)) than F8BT-SDS or PS200. The study results highlight that F8BT surface chemistry influences the composition of the protein corona, while the properties of the conjugated polymer nanoparticle surfactant stabilizer used determine particle internalization and biocompatibility profile.

AB - Conjugated polymer nanoparticles are being developed for a variety of diagnostic and theranostic applications. The conjugated polymer, F8BT, a polyfluorene derivative, was used as a model system to examine the biological behavior of conjugated polymer nanoparticle formulations stabilized with ionic (sodium dodecyl sulfate; F8BT-SDS; ∼207 nm; -31 mV) and nonionic (pegylated 12-hydroxystearate; F8BT-PEG; ∼175 nm; -5 mV) surfactants, and compared with polystyrene nanoparticles of a similar size (PS200; ∼217 nm; -40 mV). F8BT nanoparticles were as hydrophobic as PS200 (hydrophobic interaction chromatography index value: 0.96) and showed evidence of protein corona formation after incubation with serum-containing medium; however, unlike polystyrene, F8BT nanoparticles did not enrich specific proteins onto the nanoparticle surface. J774A.1 macrophage cells internalized approximately ∼20% and ∼60% of the F8BT-SDS and PS200 delivered dose (calculated by the ISDD model) in serum-supplemented and serum-free conditions, respectively, while cell association of F8BT-PEG was minimal (<5% of the delivered dose). F8BT-PEG, however, was more cytotoxic (IC50 4.5 μg cm(-2)) than F8BT-SDS or PS200. The study results highlight that F8BT surface chemistry influences the composition of the protein corona, while the properties of the conjugated polymer nanoparticle surfactant stabilizer used determine particle internalization and biocompatibility profile.

U2 - 10.1021/bm501649y

DO - 10.1021/bm501649y

M3 - Article

C2 - 25590257

VL - 16

SP - 733

EP - 742

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 3

ER -