Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics

Research output: Contribution to journalArticle

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Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics. / Ast, Julia; Arvaniti, Anastasia; Fine, Nicholas H. F.; Nasteska, Daniela; Ashford, Fiona B.; Stamataki, Zania; Koszegi, Zsombor; Bacon, Andrea; Jones, Ben J.; Lucey, Maria A.; Sasaki, Shugo; Brierley, Daniel I.; Hastoy, Benoit; Tomas, Alejandra; D’agostino, Giuseppe; Reimann, Frank; Lynn, Francis C.; Reissaus, Christopher A.; Linnemann, Amelia K.; D’este, Elisa; Calebiro, Davide; Trapp, Stefan; Johnsson, Kai; Podewin, Tom; Broichhagen, Johannes; Hodson, David J.

In: Nature Communications, Vol. 11, No. 1, 467 , 24.01.2020.

Research output: Contribution to journalArticle

Harvard

Ast, J, Arvaniti, A, Fine, NHF, Nasteska, D, Ashford, FB, Stamataki, Z, Koszegi, Z, Bacon, A, Jones, BJ, Lucey, MA, Sasaki, S, Brierley, DI, Hastoy, B, Tomas, A, D’agostino, G, Reimann, F, Lynn, FC, Reissaus, CA, Linnemann, AK, D’este, E, Calebiro, D, Trapp, S, Johnsson, K, Podewin, T, Broichhagen, J & Hodson, DJ 2020, 'Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics', Nature Communications, vol. 11, no. 1, 467 . https://doi.org/10.1038/s41467-020-14309-w

APA

Ast, J., Arvaniti, A., Fine, N. H. F., Nasteska, D., Ashford, F. B., Stamataki, Z., Koszegi, Z., Bacon, A., Jones, B. J., Lucey, M. A., Sasaki, S., Brierley, D. I., Hastoy, B., Tomas, A., D’agostino, G., Reimann, F., Lynn, F. C., Reissaus, C. A., Linnemann, A. K., ... Hodson, D. J. (2020). Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics. Nature Communications, 11(1), [467 ]. https://doi.org/10.1038/s41467-020-14309-w

Vancouver

Author

Ast, Julia ; Arvaniti, Anastasia ; Fine, Nicholas H. F. ; Nasteska, Daniela ; Ashford, Fiona B. ; Stamataki, Zania ; Koszegi, Zsombor ; Bacon, Andrea ; Jones, Ben J. ; Lucey, Maria A. ; Sasaki, Shugo ; Brierley, Daniel I. ; Hastoy, Benoit ; Tomas, Alejandra ; D’agostino, Giuseppe ; Reimann, Frank ; Lynn, Francis C. ; Reissaus, Christopher A. ; Linnemann, Amelia K. ; D’este, Elisa ; Calebiro, Davide ; Trapp, Stefan ; Johnsson, Kai ; Podewin, Tom ; Broichhagen, Johannes ; Hodson, David J. / Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics. In: Nature Communications. 2020 ; Vol. 11, No. 1.

Bibtex

@article{1578ba1243fe46c0a90f5a335291e711,
title = "Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics",
abstract = "The glucagon-like peptide-1 receptor (GLP1R) is a class B G protein-coupled receptor (GPCR) involved in metabolism. Presently, its visualization is limited to genetic manipulation, antibody detection or the use of probes that stimulate receptor activation. Herein, we present LUXendin645, a far-red fluorescent GLP1R antagonistic peptide label. LUXendin645 produces intense and specific membrane labeling throughout live and fixed tissue. GLP1R signaling can additionally be evoked when the receptor is allosterically modulated in the presence of LUXendin645. Using LUXendin645 and LUXendin651, we describe islet, brain and hESC-derived β-like cell GLP1R expression patterns, reveal higher-order GLP1R organization including membrane nanodomains, and track single receptor subpopulations. We furthermore show that the LUXendin backbone can be optimized for intravital two-photon imaging by installing a red fluorophore. Thus, our super-resolution compatible labeling probes allow visualization of endogenous GLP1R, and provide insight into class B GPCR distribution and dynamics both in vitro and in vivo.",
author = "Julia Ast and Anastasia Arvaniti and Fine, {Nicholas H. F.} and Daniela Nasteska and Ashford, {Fiona B.} and Zania Stamataki and Zsombor Koszegi and Andrea Bacon and Jones, {Ben J.} and Lucey, {Maria A.} and Shugo Sasaki and Brierley, {Daniel I.} and Benoit Hastoy and Alejandra Tomas and Giuseppe D{\textquoteright}agostino and Frank Reimann and Lynn, {Francis C.} and Reissaus, {Christopher A.} and Linnemann, {Amelia K.} and Elisa D{\textquoteright}este and Davide Calebiro and Stefan Trapp and Kai Johnsson and Tom Podewin and Johannes Broichhagen and Hodson, {David J.}",
year = "2020",
month = jan
day = "24",
doi = "10.1038/s41467-020-14309-w",
language = "English",
volume = "11",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Springer",
number = "1",

}

RIS

TY - JOUR

T1 - Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics

AU - Ast, Julia

AU - Arvaniti, Anastasia

AU - Fine, Nicholas H. F.

AU - Nasteska, Daniela

AU - Ashford, Fiona B.

AU - Stamataki, Zania

AU - Koszegi, Zsombor

AU - Bacon, Andrea

AU - Jones, Ben J.

AU - Lucey, Maria A.

AU - Sasaki, Shugo

AU - Brierley, Daniel I.

AU - Hastoy, Benoit

AU - Tomas, Alejandra

AU - D’agostino, Giuseppe

AU - Reimann, Frank

AU - Lynn, Francis C.

AU - Reissaus, Christopher A.

AU - Linnemann, Amelia K.

AU - D’este, Elisa

AU - Calebiro, Davide

AU - Trapp, Stefan

AU - Johnsson, Kai

AU - Podewin, Tom

AU - Broichhagen, Johannes

AU - Hodson, David J.

PY - 2020/1/24

Y1 - 2020/1/24

N2 - The glucagon-like peptide-1 receptor (GLP1R) is a class B G protein-coupled receptor (GPCR) involved in metabolism. Presently, its visualization is limited to genetic manipulation, antibody detection or the use of probes that stimulate receptor activation. Herein, we present LUXendin645, a far-red fluorescent GLP1R antagonistic peptide label. LUXendin645 produces intense and specific membrane labeling throughout live and fixed tissue. GLP1R signaling can additionally be evoked when the receptor is allosterically modulated in the presence of LUXendin645. Using LUXendin645 and LUXendin651, we describe islet, brain and hESC-derived β-like cell GLP1R expression patterns, reveal higher-order GLP1R organization including membrane nanodomains, and track single receptor subpopulations. We furthermore show that the LUXendin backbone can be optimized for intravital two-photon imaging by installing a red fluorophore. Thus, our super-resolution compatible labeling probes allow visualization of endogenous GLP1R, and provide insight into class B GPCR distribution and dynamics both in vitro and in vivo.

AB - The glucagon-like peptide-1 receptor (GLP1R) is a class B G protein-coupled receptor (GPCR) involved in metabolism. Presently, its visualization is limited to genetic manipulation, antibody detection or the use of probes that stimulate receptor activation. Herein, we present LUXendin645, a far-red fluorescent GLP1R antagonistic peptide label. LUXendin645 produces intense and specific membrane labeling throughout live and fixed tissue. GLP1R signaling can additionally be evoked when the receptor is allosterically modulated in the presence of LUXendin645. Using LUXendin645 and LUXendin651, we describe islet, brain and hESC-derived β-like cell GLP1R expression patterns, reveal higher-order GLP1R organization including membrane nanodomains, and track single receptor subpopulations. We furthermore show that the LUXendin backbone can be optimized for intravital two-photon imaging by installing a red fluorophore. Thus, our super-resolution compatible labeling probes allow visualization of endogenous GLP1R, and provide insight into class B GPCR distribution and dynamics both in vitro and in vivo.

U2 - 10.1038/s41467-020-14309-w

DO - 10.1038/s41467-020-14309-w

M3 - Article

C2 - 31980626

VL - 11

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 467

ER -