Sulfate and thiosulfate transport in Escherichia coli K-12: evidence for a functional overlapping of sulfate- and thiosulfate-binding proteins

A Sirko; M Zatyka; E Sadowy; D Hulanicka

doi:10.1128/jb.177.14.4134-4136.1995

Sulfate and thiosulfate transport in Escherichia coli K-12: evidence for a functional overlapping of sulfate- and thiosulfate-binding proteins

A Sirko, M Zatyka, E Sadowy, D Hulanicka

Research output: Contribution to journal › Article › peer-review

63 Citations (Scopus)

Abstract

In Escherichia coli, sulfate and thiosulfate ions are transported by an ABC-type transporter consisting of both the membrane components (the products of cysT, cysW, and cysA genes) and the periplasmic binders (the products of cysP and sbp genes). The single cysP and sbp mutants are able to utilize both sulfate and thiosulfate as a sole sulfur source, while the inactivation of both genes leads to cysteine auxotrophy resulting from the block in the transport of both ions.

Original language	English
Pages (from-to)	4134-6
Number of pages	3
Journal	Journal of Bacteriology
Volume	177
Issue number	14
DOIs	https://doi.org/10.1128/jb.177.14.4134-4136.1995
Publication status	Published - Jul 1995

Keywords

Amino Acid Sequence
Bacterial Proteins
Carrier Proteins/genetics
Cell Division
Cloning, Molecular
Escherichia coli/genetics
Escherichia coli Proteins
Molecular Sequence Data
Mutagenesis, Insertional
Periplasmic Binding Proteins
Sequence Analysis, DNA
Sulfates/metabolism
Thiosulfates/metabolism

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10.1128/jb.177.14.4134-4136.1995

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title = "Sulfate and thiosulfate transport in Escherichia coli K-12: evidence for a functional overlapping of sulfate- and thiosulfate-binding proteins",

abstract = "In Escherichia coli, sulfate and thiosulfate ions are transported by an ABC-type transporter consisting of both the membrane components (the products of cysT, cysW, and cysA genes) and the periplasmic binders (the products of cysP and sbp genes). The single cysP and sbp mutants are able to utilize both sulfate and thiosulfate as a sole sulfur source, while the inactivation of both genes leads to cysteine auxotrophy resulting from the block in the transport of both ions.",

keywords = "Amino Acid Sequence, Bacterial Proteins, Carrier Proteins/genetics, Cell Division, Cloning, Molecular, Escherichia coli/genetics, Escherichia coli Proteins, Molecular Sequence Data, Mutagenesis, Insertional, Periplasmic Binding Proteins, Sequence Analysis, DNA, Sulfates/metabolism, Thiosulfates/metabolism",

author = "A Sirko and M Zatyka and E Sadowy and D Hulanicka",

year = "1995",

month = jul,

doi = "10.1128/jb.177.14.4134-4136.1995",

language = "English",

volume = "177",

pages = "4134--6",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

number = "14",

}

TY - JOUR

T1 - Sulfate and thiosulfate transport in Escherichia coli K-12

T2 - evidence for a functional overlapping of sulfate- and thiosulfate-binding proteins

AU - Sirko, A

AU - Zatyka, M

AU - Sadowy, E

AU - Hulanicka, D

PY - 1995/7

Y1 - 1995/7

N2 - In Escherichia coli, sulfate and thiosulfate ions are transported by an ABC-type transporter consisting of both the membrane components (the products of cysT, cysW, and cysA genes) and the periplasmic binders (the products of cysP and sbp genes). The single cysP and sbp mutants are able to utilize both sulfate and thiosulfate as a sole sulfur source, while the inactivation of both genes leads to cysteine auxotrophy resulting from the block in the transport of both ions.

AB - In Escherichia coli, sulfate and thiosulfate ions are transported by an ABC-type transporter consisting of both the membrane components (the products of cysT, cysW, and cysA genes) and the periplasmic binders (the products of cysP and sbp genes). The single cysP and sbp mutants are able to utilize both sulfate and thiosulfate as a sole sulfur source, while the inactivation of both genes leads to cysteine auxotrophy resulting from the block in the transport of both ions.

KW - Amino Acid Sequence

KW - Bacterial Proteins

KW - Carrier Proteins/genetics

KW - Cell Division

KW - Cloning, Molecular

KW - Escherichia coli/genetics

KW - Escherichia coli Proteins

KW - Molecular Sequence Data

KW - Mutagenesis, Insertional

KW - Periplasmic Binding Proteins

KW - Sequence Analysis, DNA

KW - Sulfates/metabolism

KW - Thiosulfates/metabolism

U2 - 10.1128/jb.177.14.4134-4136.1995

DO - 10.1128/jb.177.14.4134-4136.1995

M3 - Article

C2 - 7608089

SN - 0021-9193

VL - 177

SP - 4134

EP - 4136

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 14

ER -

Sulfate and thiosulfate transport in Escherichia coli K-12: evidence for a functional overlapping of sulfate- and thiosulfate-binding proteins

Abstract

Keywords

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