Substitutions in the Escherichia coli RNA polymerase s70 factor that affect recognition of extended -10 elements at promoters

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@article{bcf2c34cfd754aefbd63861bd60069b0,
title = "Substitutions in the Escherichia coli RNA polymerase s70 factor that affect recognition of extended -10 elements at promoters",
abstract = "Previous work has shown that the base sequence of the DNA segment immediately upstream of the -10 hexamer at bacterial promoters (the extended -10 element) can make a significant contribution to promoter strength. Guided by recently published structural information, we used alanine scanning and suppression mutagenesis of Region 2.4 and Region 3.0 of the Escherichia coli RNA polymerase sigma(70) subunit to identify amino acid sidechains that play a role in recognition of this element. Our study shows that changes in these regions of the sigma(70) subunit can affect the recognition of different extended -10 element sequences. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.",
keywords = "bacterial transcription initiation, sigma(70) subunit, promoter recognition, RNA polymerase, extended-10 promoters",
author = "Andrew Sanderson and Jennie Mitchell and Stephen Minchin and Stephen Busby",
year = "2003",
month = jun,
day = "5",
doi = "10.1016/S0014-5793(03)00500-3",
language = "English",
volume = "544",
pages = "199--205",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1-3",

}

RIS

TY - JOUR

T1 - Substitutions in the Escherichia coli RNA polymerase s70 factor that affect recognition of extended -10 elements at promoters

AU - Sanderson, Andrew

AU - Mitchell, Jennie

AU - Minchin, Stephen

AU - Busby, Stephen

PY - 2003/6/5

Y1 - 2003/6/5

N2 - Previous work has shown that the base sequence of the DNA segment immediately upstream of the -10 hexamer at bacterial promoters (the extended -10 element) can make a significant contribution to promoter strength. Guided by recently published structural information, we used alanine scanning and suppression mutagenesis of Region 2.4 and Region 3.0 of the Escherichia coli RNA polymerase sigma(70) subunit to identify amino acid sidechains that play a role in recognition of this element. Our study shows that changes in these regions of the sigma(70) subunit can affect the recognition of different extended -10 element sequences. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

AB - Previous work has shown that the base sequence of the DNA segment immediately upstream of the -10 hexamer at bacterial promoters (the extended -10 element) can make a significant contribution to promoter strength. Guided by recently published structural information, we used alanine scanning and suppression mutagenesis of Region 2.4 and Region 3.0 of the Escherichia coli RNA polymerase sigma(70) subunit to identify amino acid sidechains that play a role in recognition of this element. Our study shows that changes in these regions of the sigma(70) subunit can affect the recognition of different extended -10 element sequences. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

KW - bacterial transcription initiation

KW - sigma(70) subunit

KW - promoter recognition

KW - RNA polymerase

KW - extended-10 promoters

UR - http://www.scopus.com/inward/record.url?scp=0038694862&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(03)00500-3

DO - 10.1016/S0014-5793(03)00500-3

M3 - Article

VL - 544

SP - 199

EP - 205

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1-3

ER -