Structure of a NHEJ Polymerase-Mediated DNA Synaptic Complex

Research output: Contribution to journalArticle

Standard

Structure of a NHEJ Polymerase-Mediated DNA Synaptic Complex. / Brissett, NC; Pitcher, RS; Juarez, R; Picher, AJ; Green, AJ; Dafforn, Timothy; Fox, GC; Blanco, L; Doherty, AJ.

In: Science, Vol. 318, 19.10.2007, p. 456-459.

Research output: Contribution to journalArticle

Harvard

Brissett, NC, Pitcher, RS, Juarez, R, Picher, AJ, Green, AJ, Dafforn, T, Fox, GC, Blanco, L & Doherty, AJ 2007, 'Structure of a NHEJ Polymerase-Mediated DNA Synaptic Complex', Science, vol. 318, pp. 456-459. https://doi.org/10.1126/science.1145112

APA

Brissett, NC., Pitcher, RS., Juarez, R., Picher, AJ., Green, AJ., Dafforn, T., Fox, GC., Blanco, L., & Doherty, AJ. (2007). Structure of a NHEJ Polymerase-Mediated DNA Synaptic Complex. Science, 318, 456-459. https://doi.org/10.1126/science.1145112

Vancouver

Brissett NC, Pitcher RS, Juarez R, Picher AJ, Green AJ, Dafforn T et al. Structure of a NHEJ Polymerase-Mediated DNA Synaptic Complex. Science. 2007 Oct 19;318:456-459. https://doi.org/10.1126/science.1145112

Author

Brissett, NC ; Pitcher, RS ; Juarez, R ; Picher, AJ ; Green, AJ ; Dafforn, Timothy ; Fox, GC ; Blanco, L ; Doherty, AJ. / Structure of a NHEJ Polymerase-Mediated DNA Synaptic Complex. In: Science. 2007 ; Vol. 318. pp. 456-459.

Bibtex

@article{acfa290a98a54fad83726d86ad4aa183,
title = "Structure of a NHEJ Polymerase-Mediated DNA Synaptic Complex",
abstract = "Nonhomologous end joining (NHEJ) is a critical DNA double-strand break (DSB) repair pathway required to maintain genome stability. Many prokaryotes possess a minimalist NHEJ apparatus required to repair DSBs during stationary phase, composed of two conserved core proteins, Ku and ligase D (LigD). The crystal structure of Mycobacterium tuberculosis polymerase domain of LigD mediating the synapsis of two noncomplementary DNA ends revealed a variety of interactions, including microhomology base pairing, mismatched and flipped-out bases, and 3' termini forming hairpin-like ends. Biochemical and biophysical studies confirmed that polymerase-induced end synapsis also occurs in solution. We propose that this DNA synaptic structure reflects an intermediate bridging stage of the NHEJ process, before end processing and ligation, with both the polymerase and the DNA sequence playing pivotal roles in determining the sequential order of synapsis and remodeling before end joining.",
author = "NC Brissett and RS Pitcher and R Juarez and AJ Picher and AJ Green and Timothy Dafforn and GC Fox and L Blanco and AJ Doherty",
year = "2007",
month = oct,
day = "19",
doi = "10.1126/science.1145112",
language = "English",
volume = "318",
pages = "456--459",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",

}

RIS

TY - JOUR

T1 - Structure of a NHEJ Polymerase-Mediated DNA Synaptic Complex

AU - Brissett, NC

AU - Pitcher, RS

AU - Juarez, R

AU - Picher, AJ

AU - Green, AJ

AU - Dafforn, Timothy

AU - Fox, GC

AU - Blanco, L

AU - Doherty, AJ

PY - 2007/10/19

Y1 - 2007/10/19

N2 - Nonhomologous end joining (NHEJ) is a critical DNA double-strand break (DSB) repair pathway required to maintain genome stability. Many prokaryotes possess a minimalist NHEJ apparatus required to repair DSBs during stationary phase, composed of two conserved core proteins, Ku and ligase D (LigD). The crystal structure of Mycobacterium tuberculosis polymerase domain of LigD mediating the synapsis of two noncomplementary DNA ends revealed a variety of interactions, including microhomology base pairing, mismatched and flipped-out bases, and 3' termini forming hairpin-like ends. Biochemical and biophysical studies confirmed that polymerase-induced end synapsis also occurs in solution. We propose that this DNA synaptic structure reflects an intermediate bridging stage of the NHEJ process, before end processing and ligation, with both the polymerase and the DNA sequence playing pivotal roles in determining the sequential order of synapsis and remodeling before end joining.

AB - Nonhomologous end joining (NHEJ) is a critical DNA double-strand break (DSB) repair pathway required to maintain genome stability. Many prokaryotes possess a minimalist NHEJ apparatus required to repair DSBs during stationary phase, composed of two conserved core proteins, Ku and ligase D (LigD). The crystal structure of Mycobacterium tuberculosis polymerase domain of LigD mediating the synapsis of two noncomplementary DNA ends revealed a variety of interactions, including microhomology base pairing, mismatched and flipped-out bases, and 3' termini forming hairpin-like ends. Biochemical and biophysical studies confirmed that polymerase-induced end synapsis also occurs in solution. We propose that this DNA synaptic structure reflects an intermediate bridging stage of the NHEJ process, before end processing and ligation, with both the polymerase and the DNA sequence playing pivotal roles in determining the sequential order of synapsis and remodeling before end joining.

U2 - 10.1126/science.1145112

DO - 10.1126/science.1145112

M3 - Article

C2 - 17947582

VL - 318

SP - 456

EP - 459

JO - Science

JF - Science

SN - 0036-8075

ER -