Structural investigations of protein-lipid complexes using neutron scattering

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Structural investigations of protein-lipid complexes using neutron scattering. / Clifton, Luke A; Hall, Stephen C L; Mahmoudi, Najet; Knowles, Timothy J; Heinrich, Frank; Lakey, Jeremy H.

In: Methods in molecular biology, Vol. 2003, 20.06.2019, p. 201-251.

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Clifton, Luke A ; Hall, Stephen C L ; Mahmoudi, Najet ; Knowles, Timothy J ; Heinrich, Frank ; Lakey, Jeremy H. / Structural investigations of protein-lipid complexes using neutron scattering. In: Methods in molecular biology. 2019 ; Vol. 2003. pp. 201-251.

Bibtex

@article{8caa9a87b47241d1b7073e7a60a35482,
title = "Structural investigations of protein-lipid complexes using neutron scattering",
abstract = "Neutron scattering has significant benefits for examining the structure of protein–lipid complexes. Cold (slow) neutrons are nondamaging and predominantly interact with the atomic nucleus, meaning that neutron beams can penetrate deeply into samples, which allows for flexibility in the design of samples studied. Most importantly, there is a strong difference in neutron scattering length (i.e., scattering power) between protium (H11, 99.98% natural abundance) and deuterium (H12 or D, 0.015%). Through the mixing of H 2O and D 2O in the samples and in some cases the deuterium labeling of the biomolecules, components within a complex can be hidden or enhanced in the scattering signal. This enables both the overall structure and the relative distribution of components within a complex to be resolved. Lipid–protein complexes are most commonly studied using neutron reflectometry (NR) and small angle neutron scattering (SANS). In this review the methodologies to produce and examine a variety of model biological membrane systems using SANS and NR are detailed. These systems include supported lipid bilayers derived from vesicle dispersions or Langmuir–Blodgett deposition, tethered bilayer systems, membrane protein–lipid complexes and polymer wrapped lipid nanodiscs. The three key stages of any SANS/NR study on model membrane systems—sample preparation, data collection, and analysis—are described together with some background on the techniques themselves. ",
keywords = "Bilayer deposition, Deuteration, Isotopic contrast variation, Nanodiscs, Neutron reflectometry, Neutron scattering, Protein–lipid complexes, SMALPs, Small-angle neutron scattering, Supported lipid bilayers, Tethered lipid bilayer membranes",
author = "Clifton, {Luke A} and Hall, {Stephen C L} and Najet Mahmoudi and Knowles, {Timothy J} and Frank Heinrich and Lakey, {Jeremy H}",
year = "2019",
month = jun,
day = "20",
doi = "10.1007/978-1-4939-9512-7_11",
language = "English",
volume = "2003",
pages = "201--251",
journal = "Methods in molecular biology",
issn = "1064-3745",
publisher = "Springer",

}

RIS

TY - JOUR

T1 - Structural investigations of protein-lipid complexes using neutron scattering

AU - Clifton, Luke A

AU - Hall, Stephen C L

AU - Mahmoudi, Najet

AU - Knowles, Timothy J

AU - Heinrich, Frank

AU - Lakey, Jeremy H

PY - 2019/6/20

Y1 - 2019/6/20

N2 - Neutron scattering has significant benefits for examining the structure of protein–lipid complexes. Cold (slow) neutrons are nondamaging and predominantly interact with the atomic nucleus, meaning that neutron beams can penetrate deeply into samples, which allows for flexibility in the design of samples studied. Most importantly, there is a strong difference in neutron scattering length (i.e., scattering power) between protium (H11, 99.98% natural abundance) and deuterium (H12 or D, 0.015%). Through the mixing of H 2O and D 2O in the samples and in some cases the deuterium labeling of the biomolecules, components within a complex can be hidden or enhanced in the scattering signal. This enables both the overall structure and the relative distribution of components within a complex to be resolved. Lipid–protein complexes are most commonly studied using neutron reflectometry (NR) and small angle neutron scattering (SANS). In this review the methodologies to produce and examine a variety of model biological membrane systems using SANS and NR are detailed. These systems include supported lipid bilayers derived from vesicle dispersions or Langmuir–Blodgett deposition, tethered bilayer systems, membrane protein–lipid complexes and polymer wrapped lipid nanodiscs. The three key stages of any SANS/NR study on model membrane systems—sample preparation, data collection, and analysis—are described together with some background on the techniques themselves.

AB - Neutron scattering has significant benefits for examining the structure of protein–lipid complexes. Cold (slow) neutrons are nondamaging and predominantly interact with the atomic nucleus, meaning that neutron beams can penetrate deeply into samples, which allows for flexibility in the design of samples studied. Most importantly, there is a strong difference in neutron scattering length (i.e., scattering power) between protium (H11, 99.98% natural abundance) and deuterium (H12 or D, 0.015%). Through the mixing of H 2O and D 2O in the samples and in some cases the deuterium labeling of the biomolecules, components within a complex can be hidden or enhanced in the scattering signal. This enables both the overall structure and the relative distribution of components within a complex to be resolved. Lipid–protein complexes are most commonly studied using neutron reflectometry (NR) and small angle neutron scattering (SANS). In this review the methodologies to produce and examine a variety of model biological membrane systems using SANS and NR are detailed. These systems include supported lipid bilayers derived from vesicle dispersions or Langmuir–Blodgett deposition, tethered bilayer systems, membrane protein–lipid complexes and polymer wrapped lipid nanodiscs. The three key stages of any SANS/NR study on model membrane systems—sample preparation, data collection, and analysis—are described together with some background on the techniques themselves.

KW - Bilayer deposition

KW - Deuteration

KW - Isotopic contrast variation

KW - Nanodiscs

KW - Neutron reflectometry

KW - Neutron scattering

KW - Protein–lipid complexes

KW - SMALPs

KW - Small-angle neutron scattering

KW - Supported lipid bilayers

KW - Tethered lipid bilayer membranes

UR - http://www.scopus.com/inward/record.url?scp=85067691999&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-9512-7_11

DO - 10.1007/978-1-4939-9512-7_11

M3 - Article

C2 - 31218621

VL - 2003

SP - 201

EP - 251

JO - Methods in molecular biology

JF - Methods in molecular biology

SN - 1064-3745

ER -