Stimulation with FITC-labeled antigens confers B cells with regulatory properties

Research output: Contribution to journalArticlepeer-review

Authors

  • Cyril Planchais
  • Sandrine Delignat
  • Shina Pashova
  • Aditi Varthaman
  • Anastas Pashov
  • Jagadeesh Bayry
  • Srinivas V. Kaveri
  • Jordan D. Dimitrov
  • Sebastien Lacroix-Desmazes

Colleges, School and Institutes

External organisations

  • Laboratoire Kastler Brossel, ENS, UPMC-Paris 6
  • Bulgarian Academy of Sciences
  • Institut Curie

Abstract

B cells with regulatory properties (Bregs) were identified in human and in mice among different B-cell subsets. Their regulatory properties rely mainly on the production of anti-inflammatory cytokines, in particular IL10, IL-35 and TGFβ, and were extensively studied in mouse models of autoimmune and inflammatory diseases. However, the exact nature of the stimulatory signals conferring regulatory properties to B cells is still not clear. We serendipitously observed that fluorescein isothiocyanate (FITC) binds to a significant proportion of naïve mouse B cells. Binding of FITC to the B-cell surface implicated at least in part the B-cell receptor. It triggered IL-10 production and allowed the endocytosis of FITC-coupled antigens followed by their presentation to CD4+ T cells. In particular, B cells incubated with FITC-OVA polarized OTII T cells towards a Tr1/Th2 phenotype in vitro. Further, the adoptive transfer of B cells incubated with FITC-labeled myelin oligodendrocyte glycoprotein peptide protected mice from experimental autoimmune encephalomyelitis, a T-cell-dependent autoimmune model. Together, the data show that FITC-stimulated B cells polarize immune responses towards Tr1/Th2 and acquire immuno-modulatory properties.

Bibliographic note

Funding Information: This work was supported by INSERM, CNRS, Sorbonne Université and by a grant from LFB (Les Ulis, France). We wish to express our gratitude to Christophe Sirac (UMR CNRS 7276/INSERM U1262, Université de Limoges) and Christian Vosshenrich ( Institut Pasteur , Paris, France) for sharing LMP2 mice and OTII mice, respectively. Publisher Copyright: © 2020 Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

Details

Original languageEnglish
Article number104151
JournalCellular Immunology
Volume355
Publication statusPublished - Sep 2020

Keywords

  • Experimental autoimmune encephalitis, IL-10, Immuno-modulatory B cells

ASJC Scopus subject areas