Stimulation of tyrosine phosphorylation without inositol lipid hydrolysis in human B lymphocytes on engaging CD72

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Stimulation of tyrosine phosphorylation without inositol lipid hydrolysis in human B lymphocytes on engaging CD72. / Kamal, Mohammed; Knox, Kirstine; Finney, Michael; Michell, Robert H.; Holder, Michelle J.; Gordon, John.

In: FEBS Letters, Vol. 319, No. 3, 22.03.1993, p. 212-216.

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@article{f856eda1b4564437ab9161bd2d206444,
title = "Stimulation of tyrosine phosphorylation without inositol lipid hydrolysis in human B lymphocytes on engaging CD72",
abstract = "Occupancy of CD72 on resting tonsillar B cells by monoclonal antibodies (mAb) promotes entry into the G1 phase of the cell cycle with an accompanying increase in MHC Class II expression and provides a co-stimulus to immobilized anti-μ for driving DNA synthesis. We now report that engagement of CD72 by mAb stimulates tyrosine phosphorylation in B cells with a peak of activity seen at 5-10 min. Two major substrates of 29 and 57 kDa showed a basal level of phosphorylation which increased with time, while a 40 kDa protein and several other minor components were phosphorylated de novo on the addition of mAb to CD72. Inositol lipid hydrolysis was found to be unperturbed, although a shallow rise in the basal level of intracellular free Ca2+ was provoked on engaging CD72. Receptor cross-linking was not a requirement for signaling human B cells through CD72: simple occupancy by univalent antibody was sufficient both to trigger the rise in basal [Ca2+]i and to promote DNA synthesis.",
keywords = "B lymphocyte, CD5, CD72, Lyb-2, Tyrosine kinase",
author = "Mohammed Kamal and Kirstine Knox and Michael Finney and Michell, {Robert H.} and Holder, {Michelle J.} and John Gordon",
year = "1993",
month = mar,
day = "22",
doi = "10.1016/0014-5793(93)80548-9",
language = "English",
volume = "319",
pages = "212--216",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Stimulation of tyrosine phosphorylation without inositol lipid hydrolysis in human B lymphocytes on engaging CD72

AU - Kamal, Mohammed

AU - Knox, Kirstine

AU - Finney, Michael

AU - Michell, Robert H.

AU - Holder, Michelle J.

AU - Gordon, John

PY - 1993/3/22

Y1 - 1993/3/22

N2 - Occupancy of CD72 on resting tonsillar B cells by monoclonal antibodies (mAb) promotes entry into the G1 phase of the cell cycle with an accompanying increase in MHC Class II expression and provides a co-stimulus to immobilized anti-μ for driving DNA synthesis. We now report that engagement of CD72 by mAb stimulates tyrosine phosphorylation in B cells with a peak of activity seen at 5-10 min. Two major substrates of 29 and 57 kDa showed a basal level of phosphorylation which increased with time, while a 40 kDa protein and several other minor components were phosphorylated de novo on the addition of mAb to CD72. Inositol lipid hydrolysis was found to be unperturbed, although a shallow rise in the basal level of intracellular free Ca2+ was provoked on engaging CD72. Receptor cross-linking was not a requirement for signaling human B cells through CD72: simple occupancy by univalent antibody was sufficient both to trigger the rise in basal [Ca2+]i and to promote DNA synthesis.

AB - Occupancy of CD72 on resting tonsillar B cells by monoclonal antibodies (mAb) promotes entry into the G1 phase of the cell cycle with an accompanying increase in MHC Class II expression and provides a co-stimulus to immobilized anti-μ for driving DNA synthesis. We now report that engagement of CD72 by mAb stimulates tyrosine phosphorylation in B cells with a peak of activity seen at 5-10 min. Two major substrates of 29 and 57 kDa showed a basal level of phosphorylation which increased with time, while a 40 kDa protein and several other minor components were phosphorylated de novo on the addition of mAb to CD72. Inositol lipid hydrolysis was found to be unperturbed, although a shallow rise in the basal level of intracellular free Ca2+ was provoked on engaging CD72. Receptor cross-linking was not a requirement for signaling human B cells through CD72: simple occupancy by univalent antibody was sufficient both to trigger the rise in basal [Ca2+]i and to promote DNA synthesis.

KW - B lymphocyte

KW - CD5

KW - CD72

KW - Lyb-2

KW - Tyrosine kinase

UR - http://www.scopus.com/inward/record.url?scp=0027410991&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(93)80548-9

DO - 10.1016/0014-5793(93)80548-9

M3 - Article

C2 - 7681409

AN - SCOPUS:0027410991

VL - 319

SP - 212

EP - 216

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 3

ER -