Stimulation of miniature end-plate potential frequency by fluoride may involve activation of protein kinase C

P E Light; S J Publicover

Stimulation of miniature end-plate potential frequency by fluoride may involve activation of protein kinase C

P E Light, S J Publicover

Biosciences

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Abstract

NaF caused a dose-dependent rise in miniature end-plate potential (MEPP) frequency at the frog neuromuscular junction. The effects on MEPP frequency of both NaF and the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) were rapidly reversed by the protein kinase C (PKC) inhibitor polymyxin B (2 microM). Theophylline augmented the response of MEPP frequency to TPA. It is concluded that the effect of fluoride on MEPP frequency may be through activation of phospholipase C and consequent PKC stimulation, and that the synergistic interaction of NaF and theophylline is consistent with such a mode of action.

Original language	English
Pages (from-to)	311-4
Number of pages	4
Journal	European Journal of Pharmacology
Volume	203
Issue number	2
Publication status	Published - 15 Oct 1991

Keywords

Animals
Drug Interactions
Neuromuscular Junction
Polymyxin B
Enzyme Activation
GTP-Binding Proteins
Diglycerides
Sodium Fluoride
Rana temporaria
Stimulation, Chemical
Motor Endplate
Protein Kinase C
Type C Phospholipases
Theophylline

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title = "Stimulation of miniature end-plate potential frequency by fluoride may involve activation of protein kinase C",

abstract = "NaF caused a dose-dependent rise in miniature end-plate potential (MEPP) frequency at the frog neuromuscular junction. The effects on MEPP frequency of both NaF and the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) were rapidly reversed by the protein kinase C (PKC) inhibitor polymyxin B (2 microM). Theophylline augmented the response of MEPP frequency to TPA. It is concluded that the effect of fluoride on MEPP frequency may be through activation of phospholipase C and consequent PKC stimulation, and that the synergistic interaction of NaF and theophylline is consistent with such a mode of action.",

keywords = "Animals, Drug Interactions, Neuromuscular Junction, Polymyxin B, Enzyme Activation, GTP-Binding Proteins, Diglycerides, Sodium Fluoride, Rana temporaria, Stimulation, Chemical, Motor Endplate, Protein Kinase C, Type C Phospholipases, Theophylline",

author = "Light, {P E} and Publicover, {S J}",

year = "1991",

month = oct,

day = "15",

language = "English",

volume = "203",

pages = "311--4",

journal = "European Journal of Pharmacology",

issn = "0014-2999",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Stimulation of miniature end-plate potential frequency by fluoride may involve activation of protein kinase C

AU - Light, P E

AU - Publicover, S J

PY - 1991/10/15

Y1 - 1991/10/15

N2 - NaF caused a dose-dependent rise in miniature end-plate potential (MEPP) frequency at the frog neuromuscular junction. The effects on MEPP frequency of both NaF and the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) were rapidly reversed by the protein kinase C (PKC) inhibitor polymyxin B (2 microM). Theophylline augmented the response of MEPP frequency to TPA. It is concluded that the effect of fluoride on MEPP frequency may be through activation of phospholipase C and consequent PKC stimulation, and that the synergistic interaction of NaF and theophylline is consistent with such a mode of action.

AB - NaF caused a dose-dependent rise in miniature end-plate potential (MEPP) frequency at the frog neuromuscular junction. The effects on MEPP frequency of both NaF and the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) were rapidly reversed by the protein kinase C (PKC) inhibitor polymyxin B (2 microM). Theophylline augmented the response of MEPP frequency to TPA. It is concluded that the effect of fluoride on MEPP frequency may be through activation of phospholipase C and consequent PKC stimulation, and that the synergistic interaction of NaF and theophylline is consistent with such a mode of action.

KW - Animals

KW - Drug Interactions

KW - Neuromuscular Junction

KW - Polymyxin B

KW - Enzyme Activation

KW - GTP-Binding Proteins

KW - Diglycerides

KW - Sodium Fluoride

KW - Rana temporaria

KW - Stimulation, Chemical

KW - Motor Endplate

KW - Protein Kinase C

KW - Type C Phospholipases

KW - Theophylline

M3 - Article

C2 - 1800124

SN - 0014-2999

VL - 203

SP - 311

EP - 314

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

IS - 2

ER -

Stimulation of miniature end-plate potential frequency by fluoride may involve activation of protein kinase C

Abstract

Keywords

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