Stimulation of macrophage growth and multinucleated cell formation in rat bone marrow cultures by insulin-like growth factor I

Research output: Contribution to journalArticlepeer-review


Colleges, School and Institutes


In this study the effects of rhIGF-I on macrophage differentiation and growth have been studied using liquid suspension cultures of rat bone marrow cells. IGF-I stimulated macrophage growth in a dose-dependent manner, a maximum response was found at a concentration of 20 ng/ml. IGF-I effects could be ascribed to stimulation of both postmitotic and proliferating cells. A remarkable finding was that IGF-I induced formation of multinucleated cells (MNC). The MNC resembled macrophage-like cells (AcP, NSE positive). A monoclonal antibody to rhIGF-I significantly inhibited IGF-stimulated macrophage growth and MNC formation. A specific antibody to mouse CSF-1 reduced IGF-stimulated macrophage growth in mouse bone marrow cultures indicating that IGF-I effects could, at least in part, be ascribed to endogenous production of CSF-1. These findings indicate that IGF-I in concert with locally induced CSF-1 can influence the differentiation and growth of bone marrow-derived macrophages.


Original languageEnglish
Pages (from-to)647-53
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 31 Jan 1991


  • Macrophages, Animals, Recombinant Proteins, Hematopoietic Stem Cells, Cell Nucleus, Insulin-Like Growth Factor I, Macrophage Colony-Stimulating Factor, Rats, Antibodies, Monoclonal, Bone Marrow Cells, Antibodies, Cells, Cultured, Kinetics, DNA Replication, Cell Division