Stabilisation of cables of fibronectin with micromolar concentrations of copper: cell substrate properties

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Stabilisation of cables of fibronectin with micromolar concentrations of copper : cell substrate properties. / Ahmed, Zubair; Briden, Anita; Hall, Susan; Brown, Robert A.

In: Biomaterials, Vol. 25, No. 5, 29.02.2004, p. 803-812.

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Ahmed, Zubair ; Briden, Anita ; Hall, Susan ; Brown, Robert A. / Stabilisation of cables of fibronectin with micromolar concentrations of copper : cell substrate properties. In: Biomaterials. 2004 ; Vol. 25, No. 5. pp. 803-812.

Bibtex

@article{5499441c2a754ae7a52da2f2bb80f924,
title = "Stabilisation of cables of fibronectin with micromolar concentrations of copper: cell substrate properties",
abstract = "We have previously described the production of large cables of fibronectin, a large extracellular matrix cell adhesion glycoprotein, which has a potential application in tissue engineering. Here we have stabilised these cables for longer survival and looked at their ultrastructural cell-substrate behaviour in vitro. Dissolution experiments showed that low concentrations of copper not only caused significant material stabilisation but left pores which could promote cell ingrowth, as we have previously reported with Fn-mats. Indeed, the greatest amount of cell ingrowth was observed for copper treated cables. Immunostaining showed S-100(+) multi-layers of cells around the edge of cables while ultrastructural analysis confirmed the presence of a mixture of fibroblasts and bipolar cells associated with fragments of basal lamina, which is a Schwann cell phenotype. Interestingly, the outermost layers of cells consisted of S-100(-) cells, presumed fibroblasts, apparently 'capping' the Schwann cells. Toxicity tests revealed that Schwann cells were only able to grow at the lowest concentration of copper used (1microM) while fibroblasts grew at all concentrations tested. These results could be used to design biomaterials with optimum properties for promoting cellular ingrowth and survival in tissue engineered grafts which may be used to improve peripheral nerve repair.",
keywords = "copper, nerve regeneration, fibroblasts, Schwarm cells, fibronectin",
author = "Zubair Ahmed and Anita Briden and Susan Hall and Brown, {Robert A.}",
year = "2004",
month = feb,
day = "29",
doi = "10.1016/S0142-9612(03)00596-9",
language = "English",
volume = "25",
pages = "803--812",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier Doyma",
number = "5",

}

RIS

TY - JOUR

T1 - Stabilisation of cables of fibronectin with micromolar concentrations of copper

T2 - cell substrate properties

AU - Ahmed, Zubair

AU - Briden, Anita

AU - Hall, Susan

AU - Brown, Robert A.

PY - 2004/2/29

Y1 - 2004/2/29

N2 - We have previously described the production of large cables of fibronectin, a large extracellular matrix cell adhesion glycoprotein, which has a potential application in tissue engineering. Here we have stabilised these cables for longer survival and looked at their ultrastructural cell-substrate behaviour in vitro. Dissolution experiments showed that low concentrations of copper not only caused significant material stabilisation but left pores which could promote cell ingrowth, as we have previously reported with Fn-mats. Indeed, the greatest amount of cell ingrowth was observed for copper treated cables. Immunostaining showed S-100(+) multi-layers of cells around the edge of cables while ultrastructural analysis confirmed the presence of a mixture of fibroblasts and bipolar cells associated with fragments of basal lamina, which is a Schwann cell phenotype. Interestingly, the outermost layers of cells consisted of S-100(-) cells, presumed fibroblasts, apparently 'capping' the Schwann cells. Toxicity tests revealed that Schwann cells were only able to grow at the lowest concentration of copper used (1microM) while fibroblasts grew at all concentrations tested. These results could be used to design biomaterials with optimum properties for promoting cellular ingrowth and survival in tissue engineered grafts which may be used to improve peripheral nerve repair.

AB - We have previously described the production of large cables of fibronectin, a large extracellular matrix cell adhesion glycoprotein, which has a potential application in tissue engineering. Here we have stabilised these cables for longer survival and looked at their ultrastructural cell-substrate behaviour in vitro. Dissolution experiments showed that low concentrations of copper not only caused significant material stabilisation but left pores which could promote cell ingrowth, as we have previously reported with Fn-mats. Indeed, the greatest amount of cell ingrowth was observed for copper treated cables. Immunostaining showed S-100(+) multi-layers of cells around the edge of cables while ultrastructural analysis confirmed the presence of a mixture of fibroblasts and bipolar cells associated with fragments of basal lamina, which is a Schwann cell phenotype. Interestingly, the outermost layers of cells consisted of S-100(-) cells, presumed fibroblasts, apparently 'capping' the Schwann cells. Toxicity tests revealed that Schwann cells were only able to grow at the lowest concentration of copper used (1microM) while fibroblasts grew at all concentrations tested. These results could be used to design biomaterials with optimum properties for promoting cellular ingrowth and survival in tissue engineered grafts which may be used to improve peripheral nerve repair.

KW - copper

KW - nerve regeneration

KW - fibroblasts

KW - Schwarm cells

KW - fibronectin

UR - http://www.scopus.com/inward/record.url?scp=0242522392&partnerID=8YFLogxK

U2 - 10.1016/S0142-9612(03)00596-9

DO - 10.1016/S0142-9612(03)00596-9

M3 - Article

C2 - 14609669

VL - 25

SP - 803

EP - 812

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 5

ER -