Stabilisation and Target Delivery of Nattokinase Using Compression Coating

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Abstract

The aim of the work is to develop a new formulation in order to stabilize a nutraceutical enzyme Nattokinase ( NKCP) in powders and to control its release rate when it passes through the gastrointestinal tract of human. NKCP powders were first compacted into a tablet, which was then coated with a mixture of an enteric material Eudragit (R) L100-55 (EL100-55) and Hydroxypropylcellulose (HPC) by direct compression. The activity of the enzyme was determined using amidolytic assay and its release rates in artificial gastric juice and an intestinal fluid were quantified using bicinchoninic acid assay. Results have shown that the activity of NKCP was pressure independent and the coated tablets protected NKCP from being denatured in the gastric juice, and realized its controlled release to the intestine based on in vitro experiments.

Details

Original languageEnglish
Pages (from-to)495-503
Number of pages9
JournalDrug Development and Industrial Pharmacy
Volume33
Issue number5
Publication statusPublished - 1 May 2007