Splenic stromal cells mediate IL-7 independent adult lymphoid tissue inducer cell survival

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@article{9f1a52696833481995234e0e54bfda89,
title = "Splenic stromal cells mediate IL-7 independent adult lymphoid tissue inducer cell survival",
abstract = "Lymphoid tissue inducer cells (LTi) play an important role in the development of lymphoid tissue in embryos. Adult CD4(+)CD3(-) LTi-like cells present a similar phenotype and gene expression to their embryonic counterpart and have important roles in CD4(+) T-cell memory and lymphoid tissue recovery following viral infection. However, adult LTi-like cells are heterogeneous populations and the factors that regulate their survival and accumulation within secondary lymphoid organs remain unclear, in particular whether the T zone stroma is involved. Here we report the identification and characterisation of a distinct subset of podoplanin(+) murine splenic stromal cells that support adult LTi-like cell survival. We have identified and isolated CD45(-)podoplanin(+) stromal cell populations which have a similar but distinct phenotype to T-zone reticular cells in lymph nodes. CD45(-)podoplanin(+) fibroblast-like cells mediate LTi-like cell survival in vitro; surprisingly this was not dependent upon IL-7 as revealed through blocking antibody experiments and studies using LTi-like cells unable to respond to {\~a} chain cytokines. Our findings show that adult LTi-like cells require extrinsic signals from podoplanin(+) splenic stromal cells to survive and suggest that IL-7 is not necessary to mediate their survival in the adult spleen.",
keywords = "Stromal cells, Spleen, Fibroblast, Lymphoid tissue",
author = "Tiezheng Hou and MZ Mustafa and Sarah Essex and Alexandra Barrington and Eric Jenkinson and Graham Anderson and Peter Lane and David Withers and Christopher Buckley",
year = "2010",
month = feb,
doi = "10.1002/eji.200939776",
language = "English",
volume = "40",
pages = "359--365",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "2",

}

RIS

TY - JOUR

T1 - Splenic stromal cells mediate IL-7 independent adult lymphoid tissue inducer cell survival

AU - Hou, Tiezheng

AU - Mustafa, MZ

AU - Essex, Sarah

AU - Barrington, Alexandra

AU - Jenkinson, Eric

AU - Anderson, Graham

AU - Lane, Peter

AU - Withers, David

AU - Buckley, Christopher

PY - 2010/2

Y1 - 2010/2

N2 - Lymphoid tissue inducer cells (LTi) play an important role in the development of lymphoid tissue in embryos. Adult CD4(+)CD3(-) LTi-like cells present a similar phenotype and gene expression to their embryonic counterpart and have important roles in CD4(+) T-cell memory and lymphoid tissue recovery following viral infection. However, adult LTi-like cells are heterogeneous populations and the factors that regulate their survival and accumulation within secondary lymphoid organs remain unclear, in particular whether the T zone stroma is involved. Here we report the identification and characterisation of a distinct subset of podoplanin(+) murine splenic stromal cells that support adult LTi-like cell survival. We have identified and isolated CD45(-)podoplanin(+) stromal cell populations which have a similar but distinct phenotype to T-zone reticular cells in lymph nodes. CD45(-)podoplanin(+) fibroblast-like cells mediate LTi-like cell survival in vitro; surprisingly this was not dependent upon IL-7 as revealed through blocking antibody experiments and studies using LTi-like cells unable to respond to ã chain cytokines. Our findings show that adult LTi-like cells require extrinsic signals from podoplanin(+) splenic stromal cells to survive and suggest that IL-7 is not necessary to mediate their survival in the adult spleen.

AB - Lymphoid tissue inducer cells (LTi) play an important role in the development of lymphoid tissue in embryos. Adult CD4(+)CD3(-) LTi-like cells present a similar phenotype and gene expression to their embryonic counterpart and have important roles in CD4(+) T-cell memory and lymphoid tissue recovery following viral infection. However, adult LTi-like cells are heterogeneous populations and the factors that regulate their survival and accumulation within secondary lymphoid organs remain unclear, in particular whether the T zone stroma is involved. Here we report the identification and characterisation of a distinct subset of podoplanin(+) murine splenic stromal cells that support adult LTi-like cell survival. We have identified and isolated CD45(-)podoplanin(+) stromal cell populations which have a similar but distinct phenotype to T-zone reticular cells in lymph nodes. CD45(-)podoplanin(+) fibroblast-like cells mediate LTi-like cell survival in vitro; surprisingly this was not dependent upon IL-7 as revealed through blocking antibody experiments and studies using LTi-like cells unable to respond to ã chain cytokines. Our findings show that adult LTi-like cells require extrinsic signals from podoplanin(+) splenic stromal cells to survive and suggest that IL-7 is not necessary to mediate their survival in the adult spleen.

KW - Stromal cells

KW - Spleen

KW - Fibroblast

KW - Lymphoid tissue

U2 - 10.1002/eji.200939776

DO - 10.1002/eji.200939776

M3 - Article

C2 - 19950181

VL - 40

SP - 359

EP - 365

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 2

ER -