Splenic stromal cells mediate IL-7 independent adult lymphoid tissue inducer cell survival
Research output: Contribution to journal › Article › peer-review
Authors
Colleges, School and Institutes
Abstract
Lymphoid tissue inducer cells (LTi) play an important role in the development of lymphoid tissue in embryos. Adult CD4(+)CD3(-) LTi-like cells present a similar phenotype and gene expression to their embryonic counterpart and have important roles in CD4(+) T-cell memory and lymphoid tissue recovery following viral infection. However, adult LTi-like cells are heterogeneous populations and the factors that regulate their survival and accumulation within secondary lymphoid organs remain unclear, in particular whether the T zone stroma is involved. Here we report the identification and characterisation of a distinct subset of podoplanin(+) murine splenic stromal cells that support adult LTi-like cell survival. We have identified and isolated CD45(-)podoplanin(+) stromal cell populations which have a similar but distinct phenotype to T-zone reticular cells in lymph nodes. CD45(-)podoplanin(+) fibroblast-like cells mediate LTi-like cell survival in vitro; surprisingly this was not dependent upon IL-7 as revealed through blocking antibody experiments and studies using LTi-like cells unable to respond to ã chain cytokines. Our findings show that adult LTi-like cells require extrinsic signals from podoplanin(+) splenic stromal cells to survive and suggest that IL-7 is not necessary to mediate their survival in the adult spleen.
Details
Original language | English |
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Pages (from-to) | 359-365 |
Number of pages | 7 |
Journal | European Journal of Immunology |
Volume | 40 |
Issue number | 2 |
Early online date | 30 Nov 2009 |
Publication status | Published - Feb 2010 |
Keywords
- Stromal cells, Spleen, Fibroblast, Lymphoid tissue