Spatiotemporal segregation of human marginal zone and memory B cell populations in lymphoid tissue

Research output: Contribution to journalArticlepeer-review


  • Yuan Zhao
  • Mohamed Uduman
  • Jacqueline HY Siu
  • Thomas Tull
  • Jeremy D Sanderson
  • Yu-Chang Bryan Wu
  • Julian Q Yhou
  • Nedyalko Petrov
  • Richard Ellis
  • Katrina Todd
  • Konstantia-Maria Chavele
  • William Guesdon
  • Wayel Jassem
  • Anna Vossenkamper
  • David P D’Cruz
  • David J Fear
  • Susan John
  • Claire Hopkins
  • Estefania Moreno
  • Natalie L Woodman
  • Francesca Ciccarelli
  • Susanne Heck
  • Steven H Kleinstein
  • Mats Bemark
  • Jo Spencer

Colleges, School and Institutes

External organisations

  • King's College London
  • Yale University
  • University of Cambridge
  • Guy's and St Thomas' NHS Foundation Trust
  • Bart's and the London School of Medicine
  • University of Gothenburg, Gothenburg, Sweden.


Human memory B cells and marginal zone (MZ) B cells share common features such as the expression of CD27 and somatic mutations in their IGHV and BCL6 genes, but the relationship between them is controversial. Here, we show phenotypic progression within lymphoid tissues as MZ B cells emerge from the mature naïve B cell pool via a precursor CD27-CD45RBMEM55+ population distant from memory cells. By imaging mass cytometry, we find that MZ B cells and memory B cells occupy different microanatomical niches in organized gut lymphoid tissues. Both populations disseminate widely between distant lymphoid tissues and blood, and both diversify their IGHV repertoire in gut germinal centres (GC), but nevertheless remain largely clonally separate. MZ B cells are therefore not developmentally contiguous with or analogous to classical memory B cells despite their shared ability to transit through GC, where somatic mutations are acquired


Original languageEnglish
Article number3857
JournalNature Communications
Publication statusPublished - 21 Sep 2018