Spatio-temporal activation of Smad1 and Smad5 in vivo: monitoring transcriptional activity of Smad proteins

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Spatio-temporal activation of Smad1 and Smad5 in vivo: monitoring transcriptional activity of Smad proteins. / Monteiro, Rui M.; Chuva de Sousa Lopes, Susana M.; Korchynskyi, Olexander; ten Dijke, Peter; Mummery, Christine L.

In: Journal of Cell Science, Vol. 117, No. 20, 15.09.2004, p. 4653-4663.

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Monteiro, Rui M. ; Chuva de Sousa Lopes, Susana M. ; Korchynskyi, Olexander ; ten Dijke, Peter ; Mummery, Christine L. / Spatio-temporal activation of Smad1 and Smad5 in vivo: monitoring transcriptional activity of Smad proteins. In: Journal of Cell Science. 2004 ; Vol. 117, No. 20. pp. 4653-4663.

Bibtex

@article{52f2a3b9475a44988b8c396017c048a0,
title = "Spatio-temporal activation of Smad1 and Smad5 in vivo:: monitoring transcriptional activity of Smad proteins",
abstract = "Signaling by bone morphogenetic proteins is essential for a wide variety of developmental processes. Receptor-regulated Smad proteins, Smads 1 and 5, are intracellular mediators of bone morphogenetic protein signaling. Together with Smad4, these proteins translocate to the nucleus and modulate transcription by binding to specific sequences on the promoters of target genes. We sought to map transcriptional Smad1/5 activity in development by generating embryonic stem cell lines carrying a Smad1/5-specific response element derived from the Id1 promoter coupled to β-galactosidase or luciferase as reporters. Three independent lines (BRE-lac1, BRE-lac2 and BRE-luc) have shown the existence of an autocrine bone morphogenetic protein signaling pathway in mouse embryonic stem cells. Reporter activity was detected in chimeric embryos, suggesting sensitivity to physiological concentrations of bone morphogenetic protein. Reporter activity in embryos from transgenic mouse lines was detected in tissues where an essential role for active bone morphogenetic protein signaling via Smads 1 or 5 had been previously established. We have thus generated, for the first time, an in vivo readout for studying the role of Smad1/5-mediated transcriptional activity in development.",
keywords = "BMP responsive element, Embryonic stem cells, Reporter mice, Smad1/5",
author = "Monteiro, {Rui M.} and {Chuva de Sousa Lopes}, {Susana M.} and Olexander Korchynskyi and {ten Dijke}, Peter and Mummery, {Christine L.}",
year = "2004",
month = sep,
day = "15",
doi = "10.1242/jcs.01337",
language = "English",
volume = "117",
pages = "4653--4663",
journal = "J. Cell Sci",
issn = "0021-9533",
publisher = "The Company of Biologists Ltd.",
number = "20",

}

RIS

TY - JOUR

T1 - Spatio-temporal activation of Smad1 and Smad5 in vivo:

T2 - monitoring transcriptional activity of Smad proteins

AU - Monteiro, Rui M.

AU - Chuva de Sousa Lopes, Susana M.

AU - Korchynskyi, Olexander

AU - ten Dijke, Peter

AU - Mummery, Christine L.

PY - 2004/9/15

Y1 - 2004/9/15

N2 - Signaling by bone morphogenetic proteins is essential for a wide variety of developmental processes. Receptor-regulated Smad proteins, Smads 1 and 5, are intracellular mediators of bone morphogenetic protein signaling. Together with Smad4, these proteins translocate to the nucleus and modulate transcription by binding to specific sequences on the promoters of target genes. We sought to map transcriptional Smad1/5 activity in development by generating embryonic stem cell lines carrying a Smad1/5-specific response element derived from the Id1 promoter coupled to β-galactosidase or luciferase as reporters. Three independent lines (BRE-lac1, BRE-lac2 and BRE-luc) have shown the existence of an autocrine bone morphogenetic protein signaling pathway in mouse embryonic stem cells. Reporter activity was detected in chimeric embryos, suggesting sensitivity to physiological concentrations of bone morphogenetic protein. Reporter activity in embryos from transgenic mouse lines was detected in tissues where an essential role for active bone morphogenetic protein signaling via Smads 1 or 5 had been previously established. We have thus generated, for the first time, an in vivo readout for studying the role of Smad1/5-mediated transcriptional activity in development.

AB - Signaling by bone morphogenetic proteins is essential for a wide variety of developmental processes. Receptor-regulated Smad proteins, Smads 1 and 5, are intracellular mediators of bone morphogenetic protein signaling. Together with Smad4, these proteins translocate to the nucleus and modulate transcription by binding to specific sequences on the promoters of target genes. We sought to map transcriptional Smad1/5 activity in development by generating embryonic stem cell lines carrying a Smad1/5-specific response element derived from the Id1 promoter coupled to β-galactosidase or luciferase as reporters. Three independent lines (BRE-lac1, BRE-lac2 and BRE-luc) have shown the existence of an autocrine bone morphogenetic protein signaling pathway in mouse embryonic stem cells. Reporter activity was detected in chimeric embryos, suggesting sensitivity to physiological concentrations of bone morphogenetic protein. Reporter activity in embryos from transgenic mouse lines was detected in tissues where an essential role for active bone morphogenetic protein signaling via Smads 1 or 5 had been previously established. We have thus generated, for the first time, an in vivo readout for studying the role of Smad1/5-mediated transcriptional activity in development.

KW - BMP responsive element

KW - Embryonic stem cells

KW - Reporter mice

KW - Smad1/5

UR - http://www.scopus.com/inward/record.url?scp=3543053797&partnerID=8YFLogxK

U2 - 10.1242/jcs.01337

DO - 10.1242/jcs.01337

M3 - Article

C2 - 15331632

AN - SCOPUS:3543053797

VL - 117

SP - 4653

EP - 4663

JO - J. Cell Sci

JF - J. Cell Sci

SN - 0021-9533

IS - 20

ER -