Sol-gel preparation of HAp-coated silica macrospheres from water glass and their protein adsorption

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Authors

Colleges, School and Institutes

External organisations

  • Okayama University

Abstract

Silica gel macrospheres of 2~4 mm in size, and wrapped with Ca-alginate chelate film were prepared as the substrate for hydroxyapatite coating layer, using water glass as the silica source. Those Ca-SiO2 macrospheres were soaked in a 1:1 (volume) mixture of ethanol and 0.1 M Na 2HPO4 to deposit hydroxyapatite layer (HAp-SiO2 macrospheres). Adsorption of bovine serum albumin and egg lysozyme on those Ca-SiO2 and HAp-SiO2 macrospheres under physiological pH (7.2) was well correlated to the Langmuir-type adsorption equation. The electrostatic interactions between the protein molecules and those macrospheres well interpret the adsorption isotherms, while the mesopores in the Ca-SiO 2contributed to some extent. A multi-layer adsorption model was proposed.

Details

Original languageEnglish
Title of host publicationBioceramics 24
Publication statusPublished - 2013
Event24th Symposium and Annual Meeting of International Society for Ceramics in Medicine, ISCM 2012 - Fukuoka, Japan
Duration: 21 Oct 201224 Oct 2012

Publication series

NameKey Engineering Materials
Number1
Volume529-530
ISSN (Print)1013-9826
ISSN (Electronic)1662-9795

Conference

Conference24th Symposium and Annual Meeting of International Society for Ceramics in Medicine, ISCM 2012
CountryJapan
CityFukuoka
Period21/10/1224/10/12

Keywords

  • Hydroxyapatite coating, Protein adsorption, Silica gel macrosphere, Water glass