SLC6A19 is a novel putative gene, induced by dioxins via AhR in human hepatoma HepG2 cells

Research output: Contribution to journalArticle

Authors

  • Wenjing Tian
  • Hualing Fu
  • Tuan Xu
  • Sherry Li Xu
  • Wuqun Tao
  • Heidi Qunhui Xie
  • Bin Zhao

Colleges, School and Institutes

External organisations

  • State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences
  • Laboratory of Immunology for Environment and Health, Shandong Analysis and Test Center, Shandong Academy of Sciences
  • University of Chinese Academy of Sciences

Abstract

The aryl hydrocarbon receptor (AhR) plays an important role in mediating dioxins toxicity. Currently, genes of P450 families are major research interests in studies on AhR-mediated gene alterations caused by dioxins. Genes related to other metabolic pathways or processes may be also responsive to dioxin exposures. Amino acid transporter B0AT1 (encoded by SLC6A19) plays a decisive role in neutral amino acid transport which is present in kidney, intestine and liver. However, effects of dioxins on its expression are still unknown. In the present study, we focused on the effects of dioxin and dioxin-like compounds on SLC6A19 expression in HepG2 cells. We identified SLC6A19 as a novel putative target gene of AhR activation in HepG2 cells. 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) increased the expression of SLC6A19 in time- and concentration-dependent manners. Using AhR antagonist CH223191 and/or siRNA assays, we demonstrated that certain AhR agonists upregulated SLC6A19 expression via AhR, including TCDD, 1,2,3,7,8-pentachlorodibenzo-p-dioxin (1,2,3,7,8-PeCDD), 2,3,4,7,8- pentachlorodibenzofuran (2,3,4,7,8-PeCDF) and PCB126. In addition, the expression of B0AT1 was also significantly induced by TCDD in HepG2 cells. Our study suggested that dioxins might affect the transcription and translation of SLC6A19 in HepG2 cells, which might be a novel putative gene to assess dioxins' toxicity in amino acid transport and metabolism in liver.

Details

Original languageEnglish
Pages (from-to)508-514
JournalEnvironmental Pollution
Volume237
Early online date15 Mar 2018
Publication statusPublished - Jun 2018

Keywords

  • dioxins, HepG2 cells, AhR, SLC6A19, B0AT1