Single-Molecule Imaging of GPCR Interactions

Research output: Contribution to journalReview article

Authors

Colleges, School and Institutes

External organisations

  • Institute of Pharmacology and Toxicology, University of Würzburg
  • Bio-Imaging Center/Rudolf Virchow Center, University of Würzburg

Abstract

G protein-coupled receptors (GPCRs) constitute the largest family of membrane receptors and are of great interest as pharmacological targets. Although the occurrence of GPCR signaling nanodomains has long been hypothesized based on indirect evidence, this and other fundamental aspects of GPCR signaling have been difficult to prove. The advent of single-molecule microscopy methods, which allow direct visualization of individual membrane proteins with unprecedented spatiotemporal resolution, provides unique opportunities to address several of these open questions. Indeed, recent single-molecule studies have revealed that GPCRs and G proteins transiently interact with each other as well as with structural components of the plasma membrane, leading to the formation of dynamic complexes and ‘hot spots’ for GPCR signaling. Whereas we are only beginning to understand the implications of this unexpected level of complexity, single-molecule approaches are likely to play a crucial role to further dissect the protein–protein interactions that are at the heart of GPCR signaling.

Details

Original languageEnglish
Pages (from-to)109-122
JournalTrends in Pharmacological Sciences
Volume39
Issue number2
Early online date17 Nov 2017
Publication statusE-pub ahead of print - 17 Nov 2017

Keywords

  • dimerization , GPCR , labeling , nanodomains , single-molecule microscopy