Single Site Discrimination of Cytosine, 5-Methylcytosine and 5-Hydroxymethylcytosine in Target DNA using Anthracene-Tagged Fluorescent Probes

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@article{1d9156da69b34edc8419094314d1d684,
title = "Single Site Discrimination of Cytosine, 5-Methylcytosine and 5-Hydroxymethylcytosine in Target DNA using Anthracene-Tagged Fluorescent Probes",
abstract = "The ability to discriminate between epigenetic variants in DNA is a necessary tool if we are to increase our understanding of the roles that they play in various biological processes and medical conditions. Herein it is demonstrated how a simple two-step fluorescent probe assay can be used to differentiate all three major epigenetic variants of cytosine at a single locus site in a target strand of DNA.",
author = "Duprey, {Jean-Louis H A} and Bullen, {Gemma A} and Zheng-Yun Zhao and Bassani, {Dario Maria} and Peacock, {Anna F A} and John Wilkie and Tucker, {James H R}",
year = "2016",
doi = "10.1021/acschembio.5b00796",
language = "English",
journal = "ACS chemical biology",
issn = "1554-8929",
publisher = "American Chemical Society",

}

RIS

TY - JOUR

T1 - Single Site Discrimination of Cytosine, 5-Methylcytosine and 5-Hydroxymethylcytosine in Target DNA using Anthracene-Tagged Fluorescent Probes

AU - Duprey, Jean-Louis H A

AU - Bullen, Gemma A

AU - Zhao, Zheng-Yun

AU - Bassani, Dario Maria

AU - Peacock, Anna F A

AU - Wilkie, John

AU - Tucker, James H R

PY - 2016

Y1 - 2016

N2 - The ability to discriminate between epigenetic variants in DNA is a necessary tool if we are to increase our understanding of the roles that they play in various biological processes and medical conditions. Herein it is demonstrated how a simple two-step fluorescent probe assay can be used to differentiate all three major epigenetic variants of cytosine at a single locus site in a target strand of DNA.

AB - The ability to discriminate between epigenetic variants in DNA is a necessary tool if we are to increase our understanding of the roles that they play in various biological processes and medical conditions. Herein it is demonstrated how a simple two-step fluorescent probe assay can be used to differentiate all three major epigenetic variants of cytosine at a single locus site in a target strand of DNA.

U2 - 10.1021/acschembio.5b00796

DO - 10.1021/acschembio.5b00796

M3 - Article

C2 - 26580817

JO - ACS chemical biology

JF - ACS chemical biology

SN - 1554-8929

ER -