Single Site Discrimination of Cytosine, 5-Methylcytosine and 5-Hydroxymethylcytosine in Target DNA using Anthracene-Tagged Fluorescent Probes

Research output: Contribution to journalArticlepeer-review

Authors

  • Gemma A Bullen
  • Zheng-Yun Zhao
  • Dario Maria Bassani

Colleges, School and Institutes

External organisations

  • Univ Bordeaux

Abstract

The ability to discriminate between epigenetic variants in DNA is a necessary tool if we are to increase our understanding of the roles that they play in various biological processes and medical conditions. Herein it is demonstrated how a simple two-step fluorescent probe assay can be used to differentiate all three major epigenetic variants of cytosine at a single locus site in a target strand of DNA.

Details

Original languageEnglish
JournalACS chemical biology
Early online date18 Nov 2015
Publication statusPublished - 2016