Shape effect of glyco-nanoparticles on macrophage cellular uptake and immune response

Research output: Contribution to journalArticlepeer-review

Authors

  • Zhen Li
  • Liang Sun
  • Yufei Zhang
  • Guosong Chen

Colleges, School and Institutes

Abstract

The shells of various poly(dl-lactide)-b-poly(acrylic acid) (PDLLA-b-PAA) spherical micelles and poly(l-lactide)-b-poly(acrylic acid) (PLLA-b-PAA) cylindrical micelles were functionalized with mannose to yield glyco-nanoparticles (GNPs) with different shapes and dimensions. All of these GNPs were shown to have good biocompatibility (up to 1 mg/mL). Cellular uptake experiments using RAW 264.7 have shown that the spherical GNPs were internalized to a much greater extent than the cylindrical GNPs and such a phenomenon was attributed to their different endocytosis pathways. It was demonstrated that spherical GNPs were internalized based on clathrin- and caveolin-mediated endocytosis while cylindrical GNPs mainly depended on clathrin-mediated endocytosis. We also found that longer cylindrical GNPs (Ln × Wn = 215 × 47 nm) can induce an inflammatory response (specifically interleukin 6) more efficiently than shorter cylindrical GNPs (Ln × Wn = 99 × 50 nm) and spherical GNPs (Dn = 46 nm).

Details

Original languageEnglish
Pages (from-to)1059-1064
JournalACS Macro Letters
Volume5
Issue number9
Early online date11 Sep 2016
Publication statusPublished - 20 Sep 2016