Serum and mucosal antibody responses to pneumococcal protein antigens in children: relationships with carriage status

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Serum and mucosal antibody responses to pneumococcal protein antigens in children : relationships with carriage status. / Zhang, Qibo; Bernatoniene, Jolanta; Bagrade, Linda; Pollard, Andrew J; Mitchell, Timothy J; Paton, James C; Finn, Adam.

In: European Journal of Immunology, Vol. 36, No. 1, 01.2006, p. 46-57.

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Zhang, Qibo ; Bernatoniene, Jolanta ; Bagrade, Linda ; Pollard, Andrew J ; Mitchell, Timothy J ; Paton, James C ; Finn, Adam. / Serum and mucosal antibody responses to pneumococcal protein antigens in children : relationships with carriage status. In: European Journal of Immunology. 2006 ; Vol. 36, No. 1. pp. 46-57.

Bibtex

@article{e9fd59815960470fbafd251674a3c18c,
title = "Serum and mucosal antibody responses to pneumococcal protein antigens in children: relationships with carriage status",
abstract = "Streptococcus pneumoniae causes significant morbidity and mortality especially in children. Some pneumococcal protein antigens can protect mice against infection. Little information is available concerning the nature of naturally acquired protective immunity to pneumococci in humans induced by these antigens. This study investigates the relationships between systemic and local antibody production and carriage in children. Children undergoing adenoidectomy (n=112, ages 2-12 years) were studied. Nasopharyngeal swabs were collected for pneumococcal culture. Serum and saliva were assayed for antibodies to several pneumococcal proteins: choline binding protein A (CbpA), pneumolysin (Ply), pneumococcal surface adhesin A (PsaA) and pneumococcal surface protein A (PspA). Adenoidal mononuclear cells (MNC) were cultured with pneumococcal culture supernatants or recombinant proteins. Cell culture supernatants were analyzed for antigen-specific antibodies. Carriage rates fell with age and serum levels of anti-CbpA, Ply and PspA rose. Anti-CbpA and -Ply serum and salivary IgG antibody levels were higher in children who were culture negative than those who were colonized. Antigen stimulation increased respective antigen-specific IgG production by adenoidal MNC and these responses were greater in those who were colonized than in culture-negative children. Antibodies to CbpA and Ply may protect children aged 2 years and older against pneumococcal colonization. Adenoids may be important local induction and effector sites for both mucosal and systemic antibody production to pneumococcal proteins in children.",
keywords = "Adenoids, Age Factors, Antibodies, Antibodies, Bacterial, Antigens, Bacterial, Blotting, Western, Child, Child, Preschool, Female, Flow Cytometry, Humans, Leukocytes, Mononuclear, Male, Nasopharynx, Respiratory Mucosa, Saliva, Streptococcus pneumoniae",
author = "Qibo Zhang and Jolanta Bernatoniene and Linda Bagrade and Pollard, {Andrew J} and Mitchell, {Timothy J} and Paton, {James C} and Adam Finn",
year = "2006",
month = jan,
doi = "10.1002/eji.200535101",
language = "English",
volume = "36",
pages = "46--57",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "1",

}

RIS

TY - JOUR

T1 - Serum and mucosal antibody responses to pneumococcal protein antigens in children

T2 - relationships with carriage status

AU - Zhang, Qibo

AU - Bernatoniene, Jolanta

AU - Bagrade, Linda

AU - Pollard, Andrew J

AU - Mitchell, Timothy J

AU - Paton, James C

AU - Finn, Adam

PY - 2006/1

Y1 - 2006/1

N2 - Streptococcus pneumoniae causes significant morbidity and mortality especially in children. Some pneumococcal protein antigens can protect mice against infection. Little information is available concerning the nature of naturally acquired protective immunity to pneumococci in humans induced by these antigens. This study investigates the relationships between systemic and local antibody production and carriage in children. Children undergoing adenoidectomy (n=112, ages 2-12 years) were studied. Nasopharyngeal swabs were collected for pneumococcal culture. Serum and saliva were assayed for antibodies to several pneumococcal proteins: choline binding protein A (CbpA), pneumolysin (Ply), pneumococcal surface adhesin A (PsaA) and pneumococcal surface protein A (PspA). Adenoidal mononuclear cells (MNC) were cultured with pneumococcal culture supernatants or recombinant proteins. Cell culture supernatants were analyzed for antigen-specific antibodies. Carriage rates fell with age and serum levels of anti-CbpA, Ply and PspA rose. Anti-CbpA and -Ply serum and salivary IgG antibody levels were higher in children who were culture negative than those who were colonized. Antigen stimulation increased respective antigen-specific IgG production by adenoidal MNC and these responses were greater in those who were colonized than in culture-negative children. Antibodies to CbpA and Ply may protect children aged 2 years and older against pneumococcal colonization. Adenoids may be important local induction and effector sites for both mucosal and systemic antibody production to pneumococcal proteins in children.

AB - Streptococcus pneumoniae causes significant morbidity and mortality especially in children. Some pneumococcal protein antigens can protect mice against infection. Little information is available concerning the nature of naturally acquired protective immunity to pneumococci in humans induced by these antigens. This study investigates the relationships between systemic and local antibody production and carriage in children. Children undergoing adenoidectomy (n=112, ages 2-12 years) were studied. Nasopharyngeal swabs were collected for pneumococcal culture. Serum and saliva were assayed for antibodies to several pneumococcal proteins: choline binding protein A (CbpA), pneumolysin (Ply), pneumococcal surface adhesin A (PsaA) and pneumococcal surface protein A (PspA). Adenoidal mononuclear cells (MNC) were cultured with pneumococcal culture supernatants or recombinant proteins. Cell culture supernatants were analyzed for antigen-specific antibodies. Carriage rates fell with age and serum levels of anti-CbpA, Ply and PspA rose. Anti-CbpA and -Ply serum and salivary IgG antibody levels were higher in children who were culture negative than those who were colonized. Antigen stimulation increased respective antigen-specific IgG production by adenoidal MNC and these responses were greater in those who were colonized than in culture-negative children. Antibodies to CbpA and Ply may protect children aged 2 years and older against pneumococcal colonization. Adenoids may be important local induction and effector sites for both mucosal and systemic antibody production to pneumococcal proteins in children.

KW - Adenoids

KW - Age Factors

KW - Antibodies

KW - Antibodies, Bacterial

KW - Antigens, Bacterial

KW - Blotting, Western

KW - Child

KW - Child, Preschool

KW - Female

KW - Flow Cytometry

KW - Humans

KW - Leukocytes, Mononuclear

KW - Male

KW - Nasopharynx

KW - Respiratory Mucosa

KW - Saliva

KW - Streptococcus pneumoniae

U2 - 10.1002/eji.200535101

DO - 10.1002/eji.200535101

M3 - Article

C2 - 16342325

VL - 36

SP - 46

EP - 57

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 1

ER -